Advertisement

Current Genetics

, Volume 34, Issue 4, pp 259–268 | Cite as

Involvement of the Saccharomyces cerevisiae UTH1 gene in the oxidative-stress response

  • Priyanka D. S. Bandara
  • Jacinta A. Flattery-O'Brien
  • Chris M. Grant
  • I. W. Dawes
ORIGINAL PAPER

Abstract

The UTH1 gene was identified by screening a Saccharomyces cerevisiae promoter-probe gene bank for oxidative stress-responsive genes. Transcription of UTH1 was decreased by the superoxide anion and increased by hydrogen peroxide. Deletion of UTH1 did not affect the growth of grande cells, however in a ρ0 background it caused retarded growth. The uth1 mutant showed increased resistance to peroxides and, in contrast, was sensitive to superoxide or the thiol oxidant diamide. Furthermore, the mutant exhibited increased survival under starvation conditions, with elevated levels of dormant cells in starved cell cultures. A multicopy plasmid containing the first half of the ORF could confer increased resistance to superoxide and increased sensitivity to peroxides/diamide/starvation on wild-type cells. The same plasmid in the uth1 background caused a highly increased mortality.

Key wordsSaccharomyces cerevisiae Oxidative stress Ageing Gene regulation 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Priyanka D. S. Bandara
    • 1
  • Jacinta A. Flattery-O'Brien
    • 1
  • Chris M. Grant
    • 1
  • I. W. Dawes
    • 1
  1. 1.Cooperative Research Centre for Food Industry Innovation and School of Biochemistry and Molecular Genetics, University of New South Wales, Sydney, NSW 2052, Australia e-mail: I.Dawes@unsw.EDU.AU Tel.: +61-2 93852089 Fax: +61-2 93851050AU

Personalised recommendations