Current Genetics

, Volume 33, Issue 4, pp 299–303

Inducing the loss of conditionally dispensable chromosomes in Nectria haematococca during vegetative growth

  • H. VanEtten
  • Sam Jorgensen
  • Jürg Enkerli
  • Sarah F. Covert
ORIGINAL PAPER

DOI: 10.1007/s002940050340

Cite this article as:
VanEtten, H., Jorgensen, S., Enkerli, J. et al. Curr Genet (1998) 33: 299. doi:10.1007/s002940050340

Abstract

A procedure for inducing and detecting the loss of conditionally dispensable (CD) chromosomes in filamentous fungi during vegetative growth was developed using Nectria haematococca mating population VI as a model. CD chromosomes in two different isolates of N. haematococca were tagged via integrative transformation with a gene conferring resistance to hygromycin B. In each case the transformation vector included chromosome-specific DNA in order to direct its homologous recombination with the desired chromosome. Chromosome loss was induced by exposing tagged isolates to inhibitory concentrations of benomyl either for protracted periods of time on solid medium or for short periods of time in liquid medium. After exposure to benomyl, isolates that lost the tagged chromosome were identified by their loss of resistance to hygromycin B. Electrophoretic karyotyping was used to verify that isolates which failed to grow on hygromycin B lacked an intact CD chromosome. Ten other chemicals known to interfere with mitotic events or cell development in other organisms did not induce CD chromosome loss in N. haematococca.

Key words Supernumerary chromosomes B chromosomes Fusarium solani Aneuploidy 

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • H. VanEtten
    • 1
  • Sam Jorgensen
    • 1
  • Jürg Enkerli
    • 2
  • Sarah F. Covert
    • 3
  1. 1.Department of Plant Pathology, University of Arizona, Tucson, AZ 85721, USA, Fax: 520-621-9290, e-mail: vanetten@ag.arizona.eduUS
  2. 2.Department of Botany, University of Georgia, Athens, GA 30602, USAGE
  3. 3.Daniel B. Warnell School of Forest Resources, University of Georgia, Athens, GA 30602, USAGE

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