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Current Genetics

, Volume 32, Issue 6, pp 408–412 | Cite as

Cloning and characterisation of the adenosyl phosphosulphate kinase gene from Aspergillus nidulans

  • David L. Clarke
  • Roger W. Newbert
  • G. Turner
ORIGINAL PAPER

Abstract

The sD gene of Aspergillus nidulans has been cloned by heterologous screening of rationally selected cosmids. Co-transformation of the sD50 mutant JMP1 confirmed the presence of a functional gene. Sequence analysis determined this gene to be 680 bp in length, containing a 59-bp intron and encoding a protein of 206 amino acids. A protein-sequence comparison revealed a similarity to the C-terminal region of ATP sulphurylase, the sC gene product. Further sequence comparison revealed differences in a consensus sequence ATP-binding motif, indicating non-functionality of the APS kinase-like domain of ATP sulphurylase, and confirms sD as the gene encoding APS kinase in A. nidulans.

Key words APS kinase ATP sulphurylase Aspergillus nidulans Allosteric inhibition 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • David L. Clarke
    • 2
  • Roger W. Newbert
    • 1
  • G. Turner
    • 1
  1. 1.Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, South Yorkshire, S10 2UH, UK Fax: ++44-114-2728697 e-mail: G.Turner@Sheffield.ac.ukGB
  2. 2.Barton Laboratory, Department of Plant Pathology, Cornell University, Geneva, NY, 14456, USAUS

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