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Current Genetics

, Volume 31, Issue 2, pp 139–143 | Cite as

Cloning and characterization of a Neurospora crassa ribosomal protein gene, crps-7

  • P. J. Vierula
ORIGINAL PAPER

Abstract

A Neurospora crassa ribosomal protein gene, crps-7, was isolated from a genomic DNA library closely linked to the morphological gene ro-2. Sequence analysis and a computerized database search revealed a high degree of homology to the Xenopus laevis rps8 and rat rps7 gene, as well as to uncharacterized ORFs from two yeast species. Comparison with a nearly full-length cDNA clone revealed two introns, one of which is in a conserved position shared with the Xenopus gene. Although a number of sequence motifs common to other N. crassa ribosomal protein genes are present upstream of the crps-7 gene, mRNA abundance is not tightly regulated by carbon availability. Relative transcript levels during nitrogen limitation and thermal stress were also examined.

Key words Ribosomal protein Fungus Neurospora crassa Regulation 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • P. J. Vierula
    • 1
  1. 1.Department of Biology, Carleton University, Ottawa, Ontario K1S 5B6, CanadaCA

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