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Current Genetics

, Volume 31, Issue 2, pp 128–132 | Cite as

Cloning the Yarrowia lipolytica homologue of the Saccharomyces cerevisiae SEC62 gene

  • D. Swennen
  • Philippe Joyet
  • Claude Gaillardin
ORIGINAL PAPER

Abstract

Yarrowia lipolytica SEC62 cDNA was cloned by functional complementation of a thermo-sensitive sec62 Saccharomyces cerevisiae mutant strain. The Y. lipolytica SEC62 promoter region was amplified by the inverse polymerase chain reaction (PCR). The cDNA codes for a 396 amino-acid protein with two potential trans-membrane domains. Y. lipolytica Sec62p behaves as an integral membrane protein as shown by Western blotting. Y. lipolytica SEC62 cDNA is able to complement a S. cerevisiae sec62 null mutant strain confirming functional conservation, although only 53.6% amino-acid similarity is observed between Y. lipolytica and S. cerevisiae Sec62.

Key wordsYarrowia lipolytica Translocation Endoplasmic reticulum Secretion 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • D. Swennen
    • 1
  • Philippe Joyet
    • 1
  • Claude Gaillardin
    • 1
  1. 1.Laboratoire de Génétique Moléculaire et Cellulaire, INRA, CNRS, INA Paris-Grignon, F-78850 Thiverval Grignon, FranceFR

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