Current Genetics

, Volume 30, Issue 5, pp 396–403

Molecular analysis of UFE1, a Saccharomyces cerevisiae gene essential for spore formation and vegetative growth

  • Thomas A. Downing
  • R. K. Storms
ORIGINAL PAPER

Abstract

 The UFE1 gene of Saccharomyces cerevisiae was cloned, sequenced and characterized. The coding region of UFE1 is separated from the TMP1 gene on chromosome XV by 624 bp. Gene-disruption experiments demonstrated that UFE1 is essential for both the germination of ascospores and for vegetative growth. Translation of the UFE1 coding region generates a protein with significant similarity to cytokeratin and to the coiled-coil region of SED5, USO1 and restin, suggesting that it is involved in the secretory pathway and may also be related to intermediate filament-associated proteins.

Key words Saccharomyces cerevisiae UFE1 Secretion TMP1 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • Thomas A. Downing
    • 1
  • R. K. Storms
    • 1
  1. 1.Department of Biology, Concordia University, 1455 De Maisonaeuve Blvd, W., Montreal H3G 1MB, CanadaCA

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