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Current Genetics

, Volume 30, Issue 2, pp 181–185 | Cite as

Occurrence of nuclear-encoded tRNAIle in mitochondria of the liverwort Marchantia polymorpha

  • Kinya Akashi
  • Katsutoshi Sakurai
  • Junya Hirayama
  • Hideya Fukuzawa
  • K. Ohyama
ORIGINAL PAPER

Abstract

 Although 29 tRNA genes have been deduced from the complete nucleotide sequence of the mitochondrial genome from the liverwort Marchantia polymorpha, a tRNAIle gene decoding AUU and AUC codons is conspicuously absent. In order to address the question of the possible involvement of nuclear-encoded tRNA, we isolated and identified three variant copies of the nuclear-encoded tRNAIle(AAU) gene from the liverwort. Northern analysis showed the presence of nuclear-encoded tRNAIle both in the mitochondrion and the cytosol, while both chloroplast DNA-encoded tRNAIle and nuclear-encoded tRNATyr were absent in liverwort mitochondria. These results unequivocally establish that import of nuclear tRNAIle into mitochondria indeed occurs in one of the most primitive plants, M. polymorpha.

Key words Marchantia polymorpha Mitochondrial tRNA tRNA import 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • Kinya Akashi
    • 1
  • Katsutoshi Sakurai
    • 1
  • Junya Hirayama
    • 1
  • Hideya Fukuzawa
    • 1
  • K. Ohyama
    • 1
  1. 1.Laboratory of Plant Molecular Biology, Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University, Kyoto, 606-01, JapanJP

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