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Current Genetics

, Volume 30, Issue 2, pp 121–125 | Cite as

Characterization of the Saccharomyces cerevisiae RTA1 gene involved in 7-aminocholesterol resistance

  • Isabelle Soustre
  • Yves Letourneux
  • F. Karst
ORIGINAL PAPER

Abstract

 7-aminocholesterol has been described as being a strong inhibitor of yeast and of Gram+-bacteria proliferation. In order to determine the precise molecular target of the toxicity of this compound, we searched for yeast resistance linked to gene over-expression. We named the new yeast gene that was isolated RTA1 (EMBL X84736). This gene led to strong resistance to the inhibitor. Gene sequencing revealed that RTA1 is adjacent to the NAB1 gene which is orientated in an opposite direction and localized on chromosome VII. The RTA1 gene, which encodes a putative protein with seven potential membrane-spanning segments, was shown to be a non-essential gene in yeast.

Key words Saccharomyces cerevisiae Aminocholesterol Ergosterol 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • Isabelle Soustre
    • 1
  • Yves Letourneux
    • 2
  • F. Karst
    • 1
  1. 1.Laboratoire de Génétique Physiologique et Moléculaire, IBMIG, 40 Avenue du Recteur Pineau, F-86022 Poitiers Cedex, FranceFR
  2. 2.Laboratoire de Physicochimie des Substances Naturelles, Université de La Rochelle, Pôle Sciences, Avenue Marillac, F-17042 La Rochelle Cedex 1, FranceFR

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