Current Genetics

, Volume 30, Issue 2, pp 115–120 | Cite as

Characterization of the Saccharomyces cerevisiaeFMS1 gene related to Candida albicans Corticosteroid-binding protein 1

  • Johann Joets
  • Dominique Pousset
  • C. Marcireau
  • F. Karst
ORIGINAL PAPER

Abstract

 In order to investigate ergosterol metabolism in S. cerevisiae we studied the CM8 mutant strain defective in the regulation of this pathway. A genomic multicopy library was screened to reverse the CM8 phenotype. This allowed us to characterize a new gene, FMS1, which relieves mutant phenotype by extragenic functional complementation. FMS1 may encode a 508 amino-acid protein. The predicted protein shares 35% identity with Cbp1p, a Candida albicans corticosteroid binding-protein. Fms1p also shows a weaker homology with monoamine oxidases. The construction of a FMS1 null-allele yeast strain demonstrated that this gene is not essential for yeast in normal usual laboratory culture conditions. The existence of a gene related to CBP1 of C. albicans in S. cerevisiae strongly suggests a possible function of steroid-binding proteins in yeast general physiology rather than in a process related to pathogenicity.

Key words Saccharomyces cerevisiae Corticosteroid-binding-protein Ergosterol metabolism 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • Johann Joets
    • 1
  • Dominique Pousset
    • 1
  • C. Marcireau
    • 1
  • F. Karst
    • 1
  1. 1.Laboratoire de Génétique Physiologique et Moléculaire, Institut de Biologie Moléculaire et d’Ingénierie Génétique, 40 avenue du Recteur Pineau, F-86022 Poitiers Cedex, FranceFR

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