Current Genetics

, Volume 39, Issue 1, pp 16–24

The chromosomal region containing pab-1, mip, and the A mating type locus of the secondarily homothallic homobasidiomycete Coprinus bilanatus

  • Ursula Kües
  • Timothy Y. James
  • Rytas Vilgalys
  • Michael P. Challen
ORIGINAL PAPER

Abstract

In this paper we describe the cloning of the DNA region containing the A1 mating type genes of the secondarily homothallic mushroom Coprinus bilanatus and compare its organization to that of heterothallic homobasidiomycetes. As in other species, the C. bilanatus A factor contains several different genes that encode two different types of homeodomain transcription factor (HD1 and HD2); and some of these genes are active in the heterologous host C. cinereus. The HD1 and HD2 genes are distributed over two closely linked subloci, and . A gene coding for a mitochondrial intermediate peptidase (mip) directly flanks the sublocus. The pab-1 gene, required for para-aminobenzoic acid synthesis, is found 39 kb upstream of mip. The structural arrangement of this chromosomal region closely resembles the heterothallic C. cinereus. In contrast, the and subloci of Schizophyllum commune are further separated, with pab-1 located between the two subloci, suggesting that a translocation event may have occurred during evolution.

Key wordsA Mating type genes Homeodomain proteins Mitochondrial intermediate peptidase gene Para-aminobenzoic acid synthase gene 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • Ursula Kües
    • 1
  • Timothy Y. James
    • 3
  • Rytas Vilgalys
    • 3
  • Michael P. Challen
    • 4
  1. 1.Institut für Mikrobiologie, ETH Zürich, Schmelzbergstrasse 7, 8092 Zürich, Switzerland e-mail: kues@micro.biol.ethz.chCH
  2. 2.Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UKGB
  3. 3.Department of Botany, Duke University, Durham, NC 27708, USAUS
  4. 4.Horticulture Research International, Wellesbourne, Warwickshire CV35 9EF, UKGB

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