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SosA in Staphylococci: an addition to the paradigm of membrane-localized, SOS-induced cell division inhibition in bacteria

  • Martin S. Bojer
  • Dorte Frees
  • Hanne IngmerEmail author
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Abstract

In all living organisms, genome replication and cell division must be coordinated to produce viable offspring. In the event of DNA damage, bacterial cells employ the SOS response to simultaneously express damage repair systems and halt cell division. Extensive characterization of SOS-controlled cell division inhibition in Escherichia coli has laid the ground for a long-standing paradigm where the cytosolic SulA protein inhibits polymerization of the central division protein, FtsZ, and thereby prevents recruitment of the division machinery at the future division site. Within the last decade, it has become clear that another, likely more general, paradigm exists, at least within the broad group of Gram-positive bacterial species, namely membrane-localized, SOS-induced cell division inhibition. We recently identified such an inhibitor in Staphylococci, SosA, and established a model for SosA-mediated cell division inhibition in Staphylococcus aureus in response to DNA damage. SosA arrests cell division subsequent to the septal localization of FtsZ and later membrane-bound division proteins, while preventing progression to septum closure, leading to synchronization of cells at this particular stage. A membrane-associated protease, CtpA negatively regulates SosA activity and likely allows growth to resume once conditions are favorable. Here, we provide a brief summary of our findings in the context of what already is known for other membrane cell division inhibitors and we emphasize how poorly characterized these intriguing processes are mechanistically. Furthermore, we put some perspective on the relevance of our findings and future developments within the field.

Keywords

DNA damage SOS response Cell division inhibition Gram-positive Staphylococcus aureus SosA CtpA 

Notes

Acknowledgements

The work in the authors’ lab was supported by grants from the Danish Council for Independent Research (1337-00129 and 1335-00772 to MSB) and the Danish National Research Foundation (DNRF120 to HI). We thank members of our own lab as well as members from the laboratories of Prof. Simon Foster (University of Sheffield) and Prof. Jan-Willem Veening (University of Lausanne) for encouraging discussions and collaboration.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Veterinary and Animal Sciences, Faculty of Health and Medical SciencesUniversity of CopenhagenCopenhagenDenmark

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