Current Genetics

, Volume 65, Issue 4, pp 857–864 | Cite as

Chaperoning RPA during DNA metabolism

  • Shuqi Li
  • Ziqi Dong
  • Shuangshuang Yang
  • Jianxun Feng
  • Qing LiEmail author


Single-stranded DNA (ssDNA) is widely generated during DNA metabolisms including DNA replication, repair and recombination and is susceptible to digestion by nucleases and secondary structure formation. It is vital for DNA metabolism and genome stability that ssDNA is protected and stabilized, which are performed by the major ssDNA-binding protein, and replication protein A (RPA) in these processes. In addition, RPA-coated ssDNA also serves as a protein–protein-binding platform for coordinating multiple events during DNA metabolisms. However, little is known about whether and how the formation of RPA–ssDNA platform is regulated. Here we highlight our recent study of a novel RPA-binding protein, Regulator of Ty1 transposition 105 (Rtt105) in Saccharomyces cerevisiae, which regulates the RPA–ssDNA platform assembly at replication forks. We propose that Rtt105 functions as an “RPA chaperone” during DNA replication, likely also promoting the assembly of RPA–ssDNA platform in other processes in which RPA plays a critical role.


RPA chaperone Rtt105 RPA–ssDNA platform DNA replication Replication stress 



This work was supported by grants from NSFC [31725015, 31830048 (QL) and 31671332 (JF)].


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© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences and Peking-Tsinghua Center for Life SciencesPeking UniversityBeijingChina
  2. 2.Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary StudiesPeking UniversityBeijingChina
  3. 3.Laboratory of Host-Pathogen BiologyThe Rockefeller UniversityNew YorkUSA

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