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FgPEX4 is involved in development, pathogenicity, and cell wall integrity in Fusarium graminearum

  • Li Zhang
  • Lina Wang
  • Yuancun LiangEmail author
  • Jinfeng YuEmail author
Original Article
  • 118 Downloads

Abstract

Peroxisomes are indispensable organelles that play critical roles in various biological processes in eukaryotic cells. PEX4, one of the peroxins, is the ubiquitin-conjugating enzyme. To functionally characterize roles of FgPEX4 in the phytopathogenic fungus, Fusarium graminearum, we constructed a deletion mutant of FgPEX4PEX4) through homologous recombination. ΔPEX4 displayed reduced mycelial growth, conidiation, and the production of perithecia. ΔPEX4 was defective in pathogenicity and production of the mycotoxin deoxynivalenol (DON). In addition, FgPEX4 was involved in cell wall integrity, lipid droplet accumulation, and the elimination of reactive oxygen species. Western blot analysis revealed reduced phosphorylation of Mgv1 in the ∆PEX4 mutant. Importantly, proteomics analysis indicated that protein expression levels related to protein biosynthesis, fatty acid metabolism, cell wall synthesis, and oxidation–reduction reactions were downregulated in ΔPEX4 compared with the wild type. Taken together, these results demonstrate that FgPEX4 is important for development, pathogenicity, and cell wall integrity.

Keywords

Fusarium graminearum FgPEX4 Peroxisome Cell wall integrity Proteomics 

Notes

Acknowledgements

We thank Larry Dunkle (Emeritus Professor, Purdue University, USA) for improving this manuscript. This work was supported by Agro-Industry R & D Special fund of China (2017YFD0201705): integration and demonstration of Chemical Fertilizer reduction Technology for Winter Wheat in East Shandong, the Wheat Innovation Team of Shandong Province Modern Agricultural Industry Technology System (SDAIT-01-09), and Funds of Shandong “Double Tops” Program (SYL2017XTTD11).

Supplementary material

294_2018_925_MOESM1_ESM.docx (1.4 mb)
Supplementary material 1 (DOCX 1481 KB)

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© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of Agricultural Microbiology, College of Plant ProtectionShandong Agricultural UniversityTai’anChina

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