Multifaceted activities of DNA polymerase η: beyond translesion DNA synthesis
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DNA polymerases are evolved to extend the 3′-OH of a growing primer annealed to a template DNA substrate. Since replicative DNA polymerases have a limited role while replicating structurally distorted template, translesion DNA polymerases mostly from Y-family come to the rescue of stalled replication fork and maintain genome stability. DNA polymerase eta is one such specialized enzyme whose function is directly associated with casual development of certain skin cancers and chemo-resistance. More than 20 years of extensive studies are available to support TLS activities of Polη in bypassing various DNA lesions, in addition, limited but crucial growing evidence also exist to suggest Polη possessing TLS-independent cellular functions. In this review, we have mostly focused on non-TLS activities of Polη from different organisms including our recent findings from pathogenic yeast Candida albicans.
KeywordsHomologous recombination Chromosomal fragile sites Antibody diversification Transcription Germ tube Candida Amphotericin B
We thank our laboratory colleagues for helpful discussions and critical comments. Special mention to Mr. Sitendra Panda for his technical assistance during the course of the study. Work in NA’s laboratory is supported by Institutional core support, DBT (BT/PR15470/MED/29/997/2015), and SERB (EMR-2016-000640). We apologize that due to space limitation, not all of the work related to this field could be discussed or cited.
- Burgers PMJ, Kunkel TA (2017) Eukaryotic DNA replication fork. Annu Rev Biochem 86:417–438. https://doi.org/10.1146/annurev-biochem-061516-044709 CrossRefPubMedPubMedCentralGoogle Scholar
- Di Noia JM, Neuberger MS (2007) Molecular mechanisms of antibody somatic hypermutation Annu Rev Biochem 76:1–22. https://doi.org/10.1146/annurev.biochem.76.061705.090740 CrossRefPubMedGoogle Scholar
- Enervald E, Lindgren E, Katou Y, Shirahige K, Strom L (2013) Importance of Poleta for damage-induced cohesion reveals differential regulation of cohesion establishment at the break site and genome-wide. PLoS Genet 9:e1003158. https://doi.org/10.1371/journal.pgen.1003158 CrossRefPubMedPubMedCentralGoogle Scholar
- Friedberg EC, Elledge SJ, Lehmann AR, Lindahl T, Muzi-Falconi M (2014) DNA repair, mutagenesis, and other responses to DNA damage. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar
- Johnson RE, Prakash S, Prakash L (1999b) Efficient bypass of a thymine-thymine dimer by yeast DNA polymerase. Poleta Sci 283:1001–1004Google Scholar
- Madril AC, Johnson RE, Washington MT, Prakash L, Prakash S (2001) Fidelity and damage bypass ability of Schizosaccharomyces pombe Eso1 protein, comprised of DNA polymerase eta and sister chromatid cohesion protein Ctf7. J Biol Chem 276:42857–42862. https://doi.org/10.1074/jbc.M106917200 CrossRefPubMedGoogle Scholar
- Nasmyth K, Haering CH (2009) Cohesin: its roles and mechanisms. Annu Rev Genet 43:525–558. https://doi.org/10.1146/annurev-genet-102108-134233 CrossRefPubMedGoogle Scholar
- Prakash S, Johnson RE, Prakash L (2005) Eukaryotic translesion synthesis DNA polymerases: specificity of structure and function. Annu Rev Biochem 74:317–353. https://doi.org/10.1146/annurev.biochem.74.082803.133250 CrossRefPubMedGoogle Scholar
- San Filippo J, Sung P, Klein H (2008) Mechanism of eukaryotic homologous recombination. Annual Rev Biochem 77:229–257. https://doi.org/10.1146/annurev.biochem.77.061306.125255 CrossRefGoogle Scholar