Involvement of the two l-lactate dehydrogenase in development and pathogenicity in Fusarium graminearum
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Lactate dehydrogenase (LDH) widely exists in organisms, which catalyzes the interconversion of pyruvate into lactate with concomitant interconversion of NADH and NAD+. In this study, two L-type lactate dehydrogenase genes FgLDHL1 and FgLDHL2 were characterized in an ascomycete fungus Fusarium graminearum, a causal agent of wheat head blight. Both the single-gene deletion mutants of FgLDHL1 or FgLDHL2 exhibited phenotypic defects in vegetative growth, sporulation, spore germination, l-lactate biosynthesis and activity. Additionally, the two l-lactate dehydrogenases were involved in the utilization of carbon sources and maintenance of redox homeostasis during spore germination. Pathogenicity assays showed that ΔFgLDHL1 exhibits reduced virulence on wheat spikelets and on corn stigmas, suggesting that it was indirectly correlated with a reduced level of deoxynivalenol accumulation. These results indicate that FgLDHL1 and FgLDHL2 play multiple roles in the developmental processes and pathogenesis in F. graminearum, and help understand the functional diversity of d-/l-lactate dehydrogenase in phytopathogenic fungi.
KeywordsFusarium graminearum l-Lactate dehydrogenase Development differentiation Deoxynivalenol Pathogenicity
This research was supported by the National Science Foundation of China (31672065) and Jiangsu Provincial Agricultural Plans [BA2018039, BE2018378 and SXGC (2016) 154].
- Genga AM, Tassi F, Lodi T, Ferrero I (1983) Mitochondrial NAD, l-lactate dehydrogenase and NAD, d-lactate dehydrogenase in the yeast Saccharomyces cerevisiae. Microbiologica 6:1Google Scholar
- Novoa WB, Winer AD, Glaid AJ, Schwert GW (1959) Lactic dehydrogenase. V. Inhibition by oxamate and by oxalate. J Biol Chem 234:1143Google Scholar
- Pizzuto R, Paventi G, Porcile C, Sarnataro D, Daniele A, Passarella S (2012) L-Lactate metabolism in HEP G2 cell mitochondria due to the l-lactate dehydrogenase determines the occurrence of the lactate/pyruvate shuttle and the appearance of oxaloacetate, malate and citrate outside mitochondria. Biochim Biophys Acta 1817:1679–1690CrossRefGoogle Scholar
- Zhang Y, He J, Jia LJ, Yuan TL, Zhang D, Guo Y, Wang Y, Tang WH (2016) Cellular tracking and gene profiling of Fusarium graminearum during maize stalk rot disease development elucidates its strategies in confronting phosphorus limitation in the host apoplast. PLoS Pathog 12:e1005485CrossRefGoogle Scholar