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Current Genetics

, Volume 65, Issue 2, pp 539–560 | Cite as

Genome sequence of the opportunistic human pathogen Magnusiomyces capitatus

  • Bronislava BrejováEmail author
  • Hana Lichancová
  • Filip Brázdovič
  • Eva Hegedűsová
  • Michaela Forgáčová Jakúbková
  • Viktória Hodorová
  • Vladimíra Džugasová
  • Andrej Baláž
  • Lucia Zeiselová
  • Andrea Cillingová
  • Martina Neboháčová
  • Vladislav Raclavský
  • Ľubomír Tomáška
  • B. Franz Lang
  • Tomáš Vinař
  • Jozef NosekEmail author
Original Article

Abstract

The yeast Magnusiomyces capitatus is an opportunistic human pathogen causing rare yet severe infections, especially in patients with hematological malignancies. Here, we report the 20.2 megabase genome sequence of an environmental strain of this species as well as the genome sequences of eight additional isolates from human and animal sources providing an insight into intraspecies variation. The distribution of single-nucleotide variants is indicative of genetic recombination events, supporting evidence for sexual reproduction in this heterothallic yeast. Using RNAseq-aided annotation, we identified genes for 6518 proteins including several expanded families such as kexin proteases and Hsp70 molecular chaperones. Several of these families are potentially associated with the ability of M. capitatus to infect and colonize humans. For the purpose of comparative analysis, we also determined the genome sequence of a closely related yeast, Magnusiomyces ingens. The genome sequences of M. capitatus and M. ingens exhibit many distinct features and represent a basis for further comparative and functional studies.

Keywords

Yeast Magnusiomyces Comparative genome analysis Pathogenicity Sexual reproduction 

Notes

Acknowledgements

We would like to thank Cletus P. Kurtzman, James Swezey (Agricultural Research Service, Peoria, IL, USA), Dominika Tanušková, and Alexandra Kolenová (University Hospital, Bratislava, Slovakia) for providing us with the yeast strains.

Funding

This research was supported by grants from the Slovak Research and Development Agency [APVV-14-0253 (JN), APVV-15-0022 (LT)], the Slovak Grant Agency [VEGA 1/0458/18 (TV), VEGA 1/0333/15 (JN), VEGA 1/0052/16 (LT), VEGA 1/0684/16 (BB)], the Comenius University grants [UK/282/2018 (HL), UK/268/2018 (VH), UK/208/2018 (LZ)], the Faculty of Medicine and Dentistry, Palacký University Olomouc [IGA_LF_2017_031 and RVO 61989592 (VR)], and NSERC Canada [RGPIN-2017-05411 (BFL)].

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Bronislava Brejová
    • 1
    Email author
  • Hana Lichancová
    • 2
  • Filip Brázdovič
    • 2
  • Eva Hegedűsová
    • 2
    • 5
  • Michaela Forgáčová Jakúbková
    • 2
  • Viktória Hodorová
    • 2
  • Vladimíra Džugasová
    • 2
  • Andrej Baláž
    • 1
  • Lucia Zeiselová
    • 2
  • Andrea Cillingová
    • 2
  • Martina Neboháčová
    • 2
  • Vladislav Raclavský
    • 3
  • Ľubomír Tomáška
    • 2
  • B. Franz Lang
    • 4
  • Tomáš Vinař
    • 1
  • Jozef Nosek
    • 2
    Email author
  1. 1.Faculty of Mathematics, Physics, and InformaticsComenius University in BratislavaBratislavaSlovakia
  2. 2.Faculty of Natural SciencesComenius University in BratislavaBratislavaSlovakia
  3. 3.Faculty of Medicine and DentistryPalacky University OlomoucOlomoucCzech Republic
  4. 4.Robert Cedergren Centre for Bioinformatics and GenomicsUniversité de MontréalMontréalCanada
  5. 5.Institute of ParasitologyBiology Centre of the Czech Academy of SciencesČeské BudějoviceCzech Republic

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