Cell size is regulated by phospholipids and not by storage lipids in Saccharomyces cerevisiae
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Cell size and morphology are key adaptive features that influence almost all aspects of cellular physiology such as cell cycle and lipid metabolism. Here we report the role of a transcription factor Suppressor Phenotype of Ty elements insertion 10 (SPT10) of Saccharomyces cerevisiae in regulating cell cycle, cell size and lipid metabolism in concert, in addition to its defined role of histone gene expression. Morphological and biochemical analyses of spt10Δ strain show an abnormal cell size, cell cycle and lipid levels. The expression of Spt10p in spt10Δ strain helps the cell revert to typical wild-type phenotypes. SPT10 controls lipid metabolism by negatively regulating the expression of lipid biosynthetic genes, and positively regulating the expression of the lipid hydrolyzing genes. Spt10p helps in maintaining the cell size by regulating the amount of carbon flux into the phospholipid constituents of the cell membranes. On the contrary, storage lipids have no role in regulating the cell size. An exogenous supply of phosphatidic acid increases the cell size, proving the positive impact of the phospholipids on cell size modulation. SPT10 affects cell cycle, cell size and lipid metabolism by an orchestrated transcriptional regulation of the corresponding genes.
KeywordsSPT10 Phosphatidic acid Phosphatidylcholine Phosphatidylethanolamine Cell size Lipid metabolism H1246 RH1246
This study was supported by the Council of Scientific and Industrial Research (CSIR), New Delhi, under the 12th 5-year plan project LIPIC (BSC0401). M. Jayaprakash Rao was supported by a fellowship from CSIR, New Delhi. The corresponding author is a recipient of the JC Bose national fellowship. We are grateful to the Department of Biochemistry, Indian Institute of Science, and C-CAMP, Bangalore for help with the radioactive study and Cell sorting facility, respectively.
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Conflict of interest
The authors declare no conflict of interest.
- Chang JS, Winston F (2013) Cell-cycle perturbations suppress the slow-growth defect of spt10Delta mutants in Saccharomyces cerevisiae. G3-Genes Genom Genet (Bethesda, MD) 3:573–583Google Scholar
- Eriksson PR, Mendiratta G, McLaughlin NB, Wolfsberg TG, Marino-Ramirez L, Pompa TA, Jainerin M, Landsman D, Shen CH, Clark DJ (2005) Global regulation by the yeast Spt10 protein is mediated through chromatin structure and the histone upstream activating sequence elements. Mol Cell Biol 25:9127–9137CrossRefPubMedCentralGoogle Scholar
- Fraser T, Gilmour A (1986) Scanning electron microscopy preparation methods: their influence on the morphology and fibril formation in Pseudomonas fragi (ATCC 4973). J Appl Microbiol 60:527–533Google Scholar
- Marty AJ, Broman AT, Zarnowski R, Dwyer TG, Bond LM, Lounes-Hadj Sahraoui A, Fontaine J, Ntambi JM, Keles S, Kendziorski C et al. (2015). Fungal morphology, iron homeostasis, and lipid metabolism regulated by a GATA transcription factor in Blastomyces dermatitidis. PLOS Pathog 11:e1004959CrossRefPubMedCentralGoogle Scholar
- Schwank S, Ebbert R, Rautenstrauss K, Schweizer E, Schuller HJ (1995) Yeast transcriptional activator INO2 interacts as an Ino2p/Ino4p basic helix-loop-helix heteromeric complex with the inositol/choline-responsive element necessary for expression of phospholipid biosynthetic genes in Saccharomyces cerevisiae. Nucleic Acids Res 23:230–237CrossRefPubMedCentralGoogle Scholar
- Szymanski KM, Binns D, Bartz R, Grishin NV, Li WP, Agarwal AK, Garg A, Anderson RG, Goodman JM (2007) The lipodystrophy protein seipin is found at endoplasmic reticulum lipid droplet junctions and is important for droplet morphology. Proc Natl Acad Sci USA 104:20890–20895CrossRefPubMedCentralGoogle Scholar