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Current Genetics

, Volume 64, Issue 5, pp 1043–1056 | Cite as

Protein O-mannosyltransferases are required for sterigmatocystin production and developmental processes in Aspergillus nidulans

  • Thi Huynh Tram Le
  • Ayana Oki
  • Masatoshi Goto
  • Kiminori Shimizu
Original Article

Abstract

Aspergillus nidulans produces sterigmatocystin (ST), a precursor of a carcinogenic secondary metabolite aflatoxin (AF), during its developmental process. ST biosynthesis has been shown to be affected by various regulatory factors. In this study, we investigated the involvement of O-mannosyltransferases (PmtA, PmtB, PmtC), in ST production and morphological development. Deletion of pmtApmtA), pmtBpmtB) or pmtCpmtC) caused no spore production and a significant decline of vegetative growth. A tremendous decline of ST level was observed in all Δpmt mutants at the third day after inoculation. By extending the growth period, ST production of ΔpmtA and ΔpmtB increased to the wild-type level 7 days after inoculation. On the other hand, ST was not detected from 7- or 14-day cultures in ΔpmtC. Expression levels of aflR gene, an essential regulator of the ST biosynthesis pathway, were also down-regulated in the Δpmt strains. By introducing the aflR overexpression cassette, ST production in the ΔpmtA and ΔpmtB significantly increased to levels comparable to the wild type. However, the presence of the aflR overexpression cassette could not improve ST production in the ΔpmtC mutant. These data suggest that the PMT family is a new endogenous factor that is required for ST biosynthesis in A. nidulans. These findings provide better understanding of the regulatory mechanisms of AF/ST biosynthesis, which can ultimately contribute to our ability to control aflatoxin contamination.

Keywords

AflR Pmt Regulation Secondary metabolite 

Notes

Acknowledgements

We are grateful to Nancy Keller of the University of Wisconsin–Madison for strains and Heng Chooi of the University of Western Australia for the plasmid pYellow. We thank Darby Brown for reviewing the manuscript before submission. This research was financially supported by the Intramural Startup Funding from the Tokyo University of Science.

Supplementary material

294_2018_816_MOESM1_ESM.docx (44 kb)
Supplementary material 1 (DOCX 44 KB)
294_2018_816_MOESM2_ESM.docx (44 kb)
Supplementary material 2 (DOCX 44 KB)
294_2018_816_MOESM3_ESM.docx (72 kb)
Supplementary material 3 (DOCX 72 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Biological Science and TechnologyTokyo University of ScienceTokyoJapan
  2. 2.Division of Microbial BiotechnologyBiotechnology Center of Ho Chi Minh CityHo Chi Minh CityVietnam
  3. 3.Faculty of AgricultureSaga UniversitySagaJapan
  4. 4.Medical Mycology Research CenterChiba UniversityChibaJapan

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