Biology and genetics of the pathogenic yeast Candida parapsilosis
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The yeast Candida parapsilosis is an opportunistic human pathogen frequently associated with nosocomial infections in neonates and patients with diminished immunity. A growing number of studies powered by recent advances in molecular genetics and genomics provide a background for uncovering the molecular basis of its virulence that suggests promising avenues for therapeutic intervention against this pathogen. Importantly, these studies also revealed several unique genetic and physiological features absent in model organisms, such as baker’s and fission yeasts. Hence, besides the clinical impact, C. parapsilosis represents an interesting non-conventional model suitable for investigations of several fundamental biological phenomena in cellular physiology, morphogenesis, and genome maintenance. In this study, we provide a concise review on C. parapsilosis biology and highlight its interesting biological features. In addition, we summarize approaches for genetic manipulation, which have enhanced research on this species by overcoming limitations of conventional genetic analysis caused primarily by an apparent absence of a sexual cycle and the diploid state of its genome.
KeywordsCandida parapsilosis Pathogenic yeast Linear mitochondrial genome Bioenergetics Morphogenesis Genetic manipulation
The authors would like to thank Ladislav Kovac (Comenius University) and Joshua D. Nosanchuk (Albert Einstein College of Medicine) for their long-term support, discussions, and critical reading of the manuscript. Our work on various aspects of C. parapsilosis biology is supported by grants from the Howard Hughes Medical Institute [55005622 (Jozef Nosek)], Fogarty International Research Collaboration Award [2-R03-TW005654-04A1 (Lubomir Tomaska)], the Slovak grant agencies APVT/APVV [0024-07 (Jozef Nosek), LPP-0164-06 (Jozef Nosek)] and VEGA [1/0219/08 (Jozef Nosek), 1/0132/09 (Lubomir Tomaska)], Janos Bólyai research fellowship (Attila Gacser), EMBO Installation Grant (Attila Gacser), and by OTKA grant no: 73250 (Attila Gacser).
- Ashford B (1928) Certain conditions of the gastro-intestinal tract in Porto Rico and their relation to tropical sprue. Am J Trop Med 8:507–538Google Scholar
- Kosa P, Valach M, Tomaska L, Wolfe KH, Nosek J (2006) Complete DNA sequences of the mitochondrial genomes of the pathogenic yeasts Candida orthopsilosis and Candida metapsilosis: insight into the evolution of linear DNA genomes from mitochondrial telomere mutants. Nucleic Acids Res 34:2472–2481PubMedCrossRefGoogle Scholar
- Langeron M, Talice R (1932) Nouvelles méthodes d’étude et essai de classification des champignons levuriformes. Ann Parasitol Hum Comp 10:1–80Google Scholar
- Middelhoven WJ, Coenen A, Kraakman B, Sollewijn Gelpke MD (1992) Degradation of some phenols and hydroxybenzoates by the imperfect ascomycetous yeasts Candida parapsilosis and Arxula adeninivorans: evidence for an operative gentisate pathway. Antonie van Leeuwenhoek 62:181–187PubMedCrossRefGoogle Scholar
- Nosek J, Tomaska L (eds) (2008) Origin and evolution of telomeres. Landes Bioscience, AustinGoogle Scholar
- Silva S, Henriques M, Martins A, Oliveira R, Williams D, Azeredo J (2009) Biofilms of non-Candida albicans Candida species: quantification, structure and matrix composition. Med Mycol. doi: 10.1080/13693780802549594
- Tavanti A, Hensgens LA, Ghelardi E, Campa M, Senesi S (2007) Genotyping of Candida orthopsilosis clinical isolates by amplification fragment length polymorphism reveals genetic diversity among independent isolates and strain maintenance within patients. J Clin Microbiol 45:1455–1462PubMedCrossRefGoogle Scholar