The dual role of autonomously replicating sequences as origins of replication and as silencers
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Autonomously replicating sequences (ARSs) in Saccharomyces cerevisiae have been extensively characterized as both origins of DNA replication and as chromatin repressors/silencers. It has been conclusively shown that the origin and the silencer activities of ARS are substantially, but not entirely interchangeable and that they are modulated by position effects and chromatin environment. It remains unclear how these two quite divergent functions of ARS co-exist. This perspective focuses on recent advances, which have shown that slight differences in ARSs can modulate their affinity for origin recognition complex and their activity as silencers or origins.
KeywordsARS DNA replication Gene silencing ORC
Autonomously replicating sequence
ARS consensus sequence
Silent information regulator
Origin recognition complex
M.A.R. and K.Y. are supported by a grant from NSERC, Canada.
- Broach JR, Li YY, Feldman J, Jayaram M, Abraham J, Nasmyth KA, Hicks JB (1983) Localization and sequence analysis of yeast origins of DNA replication. Cold Spring Harb Symp Quant Biol 47 Pt 2: 1165–1173Google Scholar
- Chang F, Theis JF, Miller J, Nieduszynski CA, Newlon CS, Weinreich M (2008) Analysis of chromosome III replicators reveals an unusual structure for the ARS318 silencer origin and a conserved WTW sequence within the origin recognition complex binding site. Mol Cell Biol 28:5071–5081. doi: 10.1128/MCB.00206-08 PubMedCrossRefGoogle Scholar
- Ramachandran L, Burhans DT, Laun P, Wang J, Liang P, Weinberger M, Wissing S, Jarolim S, Suter B, Madeo F, Breitenbach M, Burhans WC (2006) Evidence for ORC-dependent repression of budding yeast genes induced by starvation and other stresses. FEMS Yeast Res 6:763–776. doi: 10.1111/j.1567-1364.2006.00077.x PubMedCrossRefGoogle Scholar
- Rehman MA, Fourel G, Mathews A, Ramdin D, Espinosa M, Gilson E, Yankulov K (2006) Differential requirement of DNA replication factors for subtelomeric ARS consensus sequence protosilencers in Saccharomyces cerevisiae. Genetics 174:1801–1810. doi: 10.1534/genetics.106.063446 PubMedCrossRefGoogle Scholar
- Wilmes GM, Archambault V, Austin RJ, Jacobson MD, Bell SP, Cross FR (2004) Interaction of the S-phase cyclin Clb5 with an “RXL” docking sequence in the initiator protein Orc6 provides an origin-localized replication control switch. Genes Dev 18:981–991. doi: 10.1101/gad.1202304 PubMedCrossRefGoogle Scholar