Current Genetics

, Volume 52, Issue 5–6, pp 221–228

The spectrum of spontaneous mutations caused by deficiency in proteasome maturase Ump1 in Saccharomyces cerevisiae

  • Justyna McIntyre
  • Hanna Baranowska
  • Adrianna Skoneczna
  • Agnieszka Halas
  • Ewa Sledziewska-Gojska
Research Article

Abstract

Ump1 is responsible for maturation of the catalytic core of the 26S proteasome. Dysfunction of Ump1 causes an increase in the frequency of spontaneous mutations in Saccharomyces cerevisiae. In this study we analyze the spectrum of mutations occurring spontaneously in yeast deficient in Ump1 by use of the SUP4-o system. Single base substitutions predominate among the mutations analyzed (73 of the 91 alterations examined). Two major classes are GC to TA transversions and GC to AT transitions (∼50 and ∼30% of base substitutions, respectively). Besides base substitutions, almost all the major types of sequence alterations are represented. The specificity and distribution of mutations occurring in the ump1 strain are unique compared to the spectra previously established for other yeast mutators. However, the profile of mutations arising in this strain is similar to that observed in wild type. The same similarity has previously been reported for yeast deficient in Mms2, a protein involved in Rad6-dependent postreplication DNA repair (PRR). The specificity of the mutator effect caused by ump1 is discussed in light of the proposed role of the proteasome activity in the regulation of the PRR mechanisms.

Keywords

Spontaneous mutagenesis Proteasome Postreplication DNA repair Mms2 Polymerase eta 

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Justyna McIntyre
    • 1
  • Hanna Baranowska
    • 1
  • Adrianna Skoneczna
    • 1
  • Agnieszka Halas
    • 1
  • Ewa Sledziewska-Gojska
    • 1
  1. 1.Institute of Biochemistry and BiophysicsPolish Academy of SciencesWarsawPoland

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