Current Genetics

, Volume 51, Issue 6, pp 367–375 | Cite as

The fruiting-specific Le.flp1 gene, encoding a novel fungal fasciclin-like protein, of the basidiomycetous mushroom Lentinula edodes

  • Yasumasa MiyazakiEmail author
  • Shinya Kaneko
  • Masahide Sunagawa
  • Kazuo Shishido
  • Takashi Yamazaki
  • Masaya Nakamura
  • Katsuhiko Babasaki
Research Article


To understand the molecular mechanisms of fruiting body formation of basidiomycetous mushrooms, we have isolated over a 100 of developmentally regulated genes that were specifically transcribed during fruiting body development in Lentinula edodes (Shiitake-mushroom) by a subtractive hybridization, cDNA-RDA (cDNA representational difference analysis). One of these genes, named Le.flp1, was isolated from the primordial cDNA library of L. edodes, and the expression product of Le.flp1 and putative fungal homologues contained a characteristic region, homologous to the Fas domain of fasciclin family proteins, which are capable of promoting cell adhesion through Fas domain-mediated homophilic interactions in various organisms. RT-PCR analyses suggested that Le.flp1 was specifically expressed in primordia and mature fruiting bodies. In situ hybridization indicated that Le.flp1 transcripts were distributed distinctly in the following tissues: the inside of gills of fruiting bodies, especially at the boundary between the subhymenium and trama, where there is active proliferation of basidium cells for producing basidiospores; peripheral regions of the primordium, pileus and stipe; and both inner tissue and outer regions of the stipe. Our results suggest the hypothesis that Le.flp1 plays a role in cellular differentiation and development in ubiquitous tissues during fruiting body formation in L. edodes, possibly through cell adhesion.


Basidiomycete Mushroom Lentinula edodes Fruiting body formation In situ hybridization Fasciclin 



This work was supported by a Grant-in-Aid for Scientific Research (No. 17780068) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. The authors deeply appreciate Dr. Teruko Konishi, of the University of Ryukyus’ Faculty of Agriculture, for her useful and accurate comments on the experiments.


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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Yasumasa Miyazaki
    • 1
    Email author
  • Shinya Kaneko
    • 2
  • Masahide Sunagawa
    • 1
  • Kazuo Shishido
    • 2
  • Takashi Yamazaki
    • 2
    • 3
  • Masaya Nakamura
    • 1
  • Katsuhiko Babasaki
    • 1
  1. 1.Department of Applied MicrobiologyForestry and Forest Products Research InstituteTsukuba-NorinJapan
  2. 2.Department of Life Science, Graduate School of Bioscience and BiotechnologyTokyo Institute of TechnologyMidori-kuJapan
  3. 3.Institute of Space and Astronautical ScienceJapan Aerospace Exploration AgencyTsukubaJapan

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