Current Genetics

, Volume 50, Issue 1, pp 32–44

Transcriptome analysis of Aspergillus fumigatus exposed to voriconazole

  • Márcia Eliana da Silva Ferreira
  • Iran Malavazi
  • Marcela Savoldi
  • Axel A. Brakhage
  • Maria Helena S. Goldman
  • H. Stanley Kim
  • William C. Nierman
  • Gustavo H. Goldman
Research Article

DOI: 10.1007/s00294-006-0073-2

Cite this article as:
Ferreira, M.E..S., Malavazi, I., Savoldi, M. et al. Curr Genet (2006) 50: 32. doi:10.1007/s00294-006-0073-2

Abstract

For a comprehensive evaluation of genes that have their expression modulated during exposure of the mycelia to voriconazole, we performed a large-scale analysis of gene expression in Aspergillus fumigatus using a microarray hybridization approach. By comparing the expression of genes between the reference time and after addition of voriconazole (30, 60, 120, and 240 min), we identified 2,271 genes differentially expressed in the wild-type strain. To validate the expression of some of these genes during exposure to voriconazole, we analyzed 13 genes showing higher expression in the presence of voriconazole by real-time RT-PCR. Although the magnitudes of induction differed between the two experimental systems, in about 85% of the cases they were in good agreement with the microarray data. To our knowledge this is the first study of microarray hybridization analysis for a filamentous fungus exposed to an antifungal agent. In our study, we have observed: (i) a decreased mRNA expression of various ergosterol biosynthesis genes; (ii) increased mRNA levels of genes involved in a variety of cell functions, such as transporters, transcription factors, proteins involved in cell metabolism, and hypothetical proteins; and (iii) the involvement of the cyclic AMP-protein kinase signaling pathway in the increased mRNA expression of several of these genes.

Keywords

Aspergillus fumigatus Voriconazole Microarrays Transcriptome 

Supplementary material

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Márcia Eliana da Silva Ferreira
    • 1
  • Iran Malavazi
    • 1
  • Marcela Savoldi
    • 1
  • Axel A. Brakhage
    • 2
  • Maria Helena S. Goldman
    • 3
  • H. Stanley Kim
    • 4
    • 5
  • William C. Nierman
    • 4
    • 5
  • Gustavo H. Goldman
    • 1
  1. 1.Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão PretoUniversidade de São PauloRibeirão PretoBrazil
  2. 2.Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research, Infection Biology-HansKnoell Institute (HKI)University of JenaJenaGermany
  3. 3.Faculdade de Filosofia, Ciências e Letras de Ribeirão PretoUniversidade de São PauloRibeirão PretoBrazil
  4. 4.The Institute for Genomic ResearchRockvilleUSA
  5. 5.Department of Biochemistry and Molecular BiologyThe George Washington University School of MedicineWashingtonUSA

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