Moc3, a novel Zn finger type protein involved in sexual development, ascus formation, and stress response of Schizosaccharomyces pombe
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The cAMP pathway in Schizosaccharomyces pombe is the major nutrient sensing pathway to initiate sexual development when opposite mating type cells exist. We identified moc1–moc4 as genes that overcome a partially sterile S. pombe strain due to an elevation of cAMP. When we compared the strength of inducing ability of sexual development in the same S. pombe strain, Moc1 had highest, Moc2 had lowest, and both Moc3 and Moc4 had intermediate effects. Moc1/Sds23 and Moc2/Ded1 are known to be a potential regulator of M-phase progression and an essential RNA helicase, respectively. While Moc4 was found to be identical with a Zn-finger protein Zfs1, Moc3 (SPAC821.07c) was a novel protein containing a Zn-finger (Zn(2)-Cys(6)) motif. Deletion mutant of the moc3 gene was constructed and its disruptant was found to be lower in mating efficiency and formed aberrant asci. In addition, unexpectedly, a moc3 disruptant was sensitive to CaCl2 and DNA damaging agents such as MMS and UV. Those phenotypes were opposite to the phenotypes observed in a zfs1 disruptant, and quite different from the ones in a moc1 disruptant. Moc3 localized in the nucleus as observed for Zfs1. Moc3 bound with Moc4/Zfs1 weakly in the two hybrid system, but no other combination of Moc(s) bound each other in the same analysis. Thus, Moc3 is not only involved in sexual development, but also in ascus formation and DNA integrity in an independent manner with Moc1 and Moc2 in S. pombe.
KeywordsSchizosaccharomyces pombe Moc3 Cyr1 Meiosis
We thank C. Hoffman for critical reading and corrections of this manuscript. We thank J. Kanoh and M. Yamamoto for strains. This research was supported by grant-aid from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
- Alfa C, Fantes P, Hyams J, Mcleod M, Warbrick E (1993) Experiments with fission yeast: a laboratory course manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, pp 133–136Google Scholar
- Beltraminelli N, Murone M, Simanis V (1999) The S. pombe zfs1 gene is required to prevent septation if mitotic progression is inhibited. J Cell Sci 18:3103–3114Google Scholar
- Burke D, Dawson D, Stearns T (2000) Methods in yeast genetics; a Cold Spring Harbor Laboratory course manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor pp 171–182Google Scholar
- Gietz RD, Woods RA (1994) High efficiency transformation in yeast, In: Johnson JA (eds) Molecular genetics of yeast: practical approaches. Oxford University Press, Oxford, pp 121–134Google Scholar
- Goldar MM, Nishie T, Ishikura Y, Fukuda T, Takegawa K, Kawamukai M (2005) Functional conservation between fission yeast moc1/sds23 and its two orthologs, budding yeast SDS23 and SDS24, and phenotypic differences in their disruptants. Biosci Biotechnol Biochem 69:1422–1426CrossRefPubMedGoogle Scholar
- Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring HarborGoogle Scholar
- Yakura M, Ozoe F, Ishida H, Nakagawa T, Tanaka K, Matsuda H, Kawamukai M (2005) zds1, a novel gene encoding an ortholog of Zds1 and Zds2, controls sexual differentiation, cell wall integrity, and cell morphology in fission yeast. Genetics (in press)Google Scholar
- Yamamoto M (2003) Initiation of meiosis. In: Egel R (ed) The molecular biology of Schizosaccharomyces pombe. Springer, Berlin Heidelberg New York, pp 297–309Google Scholar
- Yamamoto M, Imai Y, Watanabe Y (1997) Mating and sporulation in Schizosaccharomyces pombe, In: Pringle JR, Broach JR, Jones EW (eds) The molecular and cellular biology of the yeast Saccharomyces. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, pp 1037–1106Google Scholar