Current Genetics

, Volume 46, Issue 2, pp 103–114 | Cite as

The mitochondrial plasmid of the true slime mold Physarum polycephalum bypasses uniparental inheritance by promoting mitochondrial fusion

  • Rakusa Sakurai
  • Hideo Nomura
  • Yohsuke Moriyam
  • Shigeyuki Kawano
Research Article
First Online:
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Revised:
Accepted:

Abstract

Mitochondrial DNA (mtDNA) is inherited maternally in most eukaryotes. Linear mitochondrial plasmids in higher plants and fungi are also transmitted from the maternal parent to the progeny. However, mF, which is a mitochondrial linear plasmid of Physarum polycephalum, evades uniparental mitochondrial inheritance. We examined 36 myxamoebal strains of Physarum and isolated three novel mF+ strains (JE8, TU111, NG111) that harbored free mF plasmids. These strains were mated with the mF− strain KM88. Of the three mF− × mF+ crosses, only KM88 × JE8 displayed complete uniparental inheritance. However, in KM88 × TU111 and KM88 × NG111, the mtDNA of KM88 and mF of TU111 and NG111 were inherited by the plasmodia and showed recombination. For example, although the mtDNA of TU111 was eliminated, the mF of TU111 persisted and became inserted into the mtDNA of KM88, such that recombinant mtDNA represented 80% of the total mtDNA. The parental mitochondria fused to yield giant mitochondria with two or more mitochondrial nucleoids. The mF appears to exchange mitochondria from the recipient (paternal) to the donor (maternal) by promoting mitochondrial fusion.

Keywords

Mitochondria Fusion Plasmid Uniparental inheritance Physarum polycephalum 
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Notes

Acknowledgements

We would like to thank Prof. T. Kuroiwa (Department of Life Science, College of Science, Rikkyo University) for helpful discussion and Dr. H. Takano (Department of Biological Science, Faculty of Science, Kumamoto University) for helpful technical advice. This study was supported by grants to S.K. for Scientific Research in Priority Areas (nos. 13440246, 15370027) from the Ministry of Education, Culture, Sports, Science, and Technology, Japan.

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Rakusa Sakurai
    • 1
  • Hideo Nomura
    • 1
  • Yohsuke Moriyam
    • 1
  • Shigeyuki Kawano
    • 1
  1. 1.Laboratory of Plant Life System, Department of Integrated Biosciences, Graduate School of Frontier SciencesUniversity of TokyoChibaJapan

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