Current Genetics

, Volume 46, Issue 1, pp 20–28 | Cite as

Saccharomyces cerevisiae translational activator Cbs2p is associated with mitochondrial ribosomes

  • Udo Krause-Buchholz
  • Kathrin Barth
  • Cora Dombrowski
  • Gerhard Rödel
Research Article

Abstract

A characteristic feature of the mitochondrial expression system in Saccharomyces cerevisiae is the requirement for gene-specific translational activator proteins. Translation of mitochondrial apocytochrome b mRNA requires the nucleus-encoded proteins Cbs1p and Cbs2p. These proteins are thought to tether cytochrome b mRNA to the mitochondrial inner membrane via binding to the 5′ untranslated mRNA leader. Here, we demonstrate by the use of affinity chromatography and coimmunoprecipitation that Cbs2p interacts with the mitoribosomes. We further provide evidence that the C-terminus of Cbs2p is important for ribosome association, while the N-terminal portion is essential for the formation of homomeric structures.

Keywords

Saccharomyces cerevisiae Translational activator proteins Cbs2p Mitochondrial ribosomes 

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Udo Krause-Buchholz
    • 1
  • Kathrin Barth
    • 1
    • 2
  • Cora Dombrowski
    • 1
  • Gerhard Rödel
    • 1
  1. 1.Institut für GenetikTechnische Universität DresdenDresdenGermany
  2. 2.Institut für AnatomieMedizinische Fakultät Carl Gustav CarusDresdenGermany

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