Current Genetics

, Volume 45, Issue 6, pp 390–398 | Cite as

Shuttle vectors for Candida albicans: control of plasmid copy number and elevated expression of cloned genes

  • Wenjin Du
  • Melisa Coaker
  • Jack D. Sobel
  • Robert A. Akins
Technical Note


Plasmids containing the inosine monophosphate dehydrogenase gene CaIMH3 from Candida albicans strain ATCC 32354 transform their host to resistance against mycophenolic acid (MPA). The transformants maintain the plasmids at a high copy number (20–40 per cell) and express the CaIMH3 gene at very high levels relative to untransformed controls. The plasmid copy number can be controlled by the concentration of MPA in the media. The transformation procedure is reproducible and the efficiency of transformation is high, up to 15,000 per microgram. Unrearranged plasmids are readily recovered by transforming total DNA from transformants back into Escherichia coli. C. albicans genes cloned into the plasmid are expressed at elevated levels relative to untransformed controls. A derivative vector containing the CaMAL2 promoter and termination sequences expresses the CaERG11 ORF at high levels and confers moderate resistance to fluconazole. These shuttle vectors should facilitate global genomics approaches in C. albicans that have been hampered by its diploid genome.


Candida albicans CaIMH3 Mycophenolic acid Shuttle vector 


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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Wenjin Du
    • 1
  • Melisa Coaker
    • 2
  • Jack D. Sobel
    • 2
  • Robert A. Akins
    • 1
  1. 1.Department of Biochemistry and Molecular biologyWayne State University School of MedicineDetroitUSA
  2. 2.Division of Infectious Diseases, Department of Internal MedicineWayne State University School of MedicineDetroitUSA

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