Current Genetics

, Volume 45, Issue 4, pp 214–224 | Cite as

Analysis of a nonribosomal peptide synthetase gene from Alternaria brassicae and flanking genomic sequences

  • Thomas Guillemette
  • Adnane Sellam
  • Philippe Simoneau
Research Article

Abstract

Very little information is currently available concerning the pathogenic determinants produced by Alternaria brassicae, the causal agent of the blackspot disease of crucifers. We screened a genomic library of this fungus and identified a nonribosomal peptide synthetase (NRPS) gene named AbrePsy1. The complete coding sequence is 22 kbp long and encodes a large protein (792 kDa) showing typical NRPS modular organization. Structural analysis of AbrePsy1 revealed four complete elongation modules, two of which have epimerization domains. In the vicinity of AbrePsy1, a second gene (named AbreAtr1), which encodes an ATP-binding cassette transporter was identified. Increased expression of AbrePsy1 and AbreAtr1 was observed during host-plant infection. However, while physically linked, these two genes are probably not functionally clustered, as their expression patterns differed.

Keywords

Alternaria brassicae Seed-borne pathogen Pathogenic determinant ABC transporter Nonribosomal peptide synthetase Siderophore 

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Thomas Guillemette
    • 1
  • Adnane Sellam
    • 1
  • Philippe Simoneau
    • 1
  1. 1.Faculté des SciencesUMR PAVE 77AngersFrance

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