Current Genetics

, Volume 44, Issue 3, pp 124–131 | Cite as

The Aa-Pri4 gene, specifically expressed during fruiting initiation in the Agrocybe aegerita complex, contains an unusual CT-rich leader intron within the 5′ uncoding region.

  • Pascal Sirand-Pugnet
  • Cruz Santos
  • Jacques LabarèreEmail author
Research Article


The Aa1-Pri4 gene was cloned from the edible mushroom Agrocybe aegerita. The gene, specifically expressed during fruiting initiation, encodes a glycine-rich protein of 116 amino acids, with no homology to already known proteins. Homologous genes were amplified from two other strains belonging to the Agr. aegerita complex and originating from South-East Asia; and a comparison of the three genes revealed a high conservation of the coding sequences (72.8–97.8%). The PRI4 putative protein sequences were highly similar (87.5–100.0%); and all of them contained two protein kinase C sites, suggesting a potential supplementary regulation by phosphorylation at the protein level. The 5′ uncoding regions all presented a leader intron, very variable in sequence (45.7% identity), but with a high C+T content (74.5–79.0%). The presence of such CT-rich sequences previously described in the promoter of highly expressed fungal genes suggests that the leader intron of the Aa1-Pri4 gene could be involved in the high-level, stage-specific expression.


Basidiomycota Fungi Fruiting initiation Leader intron CT-rich sequence 



This research was supported by grants from the European Community (Fonds Européen de Développement Régional), the Conseil Scientifique de l′Université Victor Ségalen Bordeaux 2, Monsieur le Préfet de la Région Aquitaine Préfet de la Gironde (Fonds National d′Aménagement et de Développement du Territoire), the Conseil Régional d′Aquitaine and the Institut National de la Recherche Agronomique. We thank Prof. Jorge Wright and Dr. E. Alberto (Argentina), Dr. R. de Leon (Guatemala) and Dr. P. Pitakpaivan (Thailand) for kindly providing strains, and S. Chideh Soliman, C. Mulot and A.S. Scheuer for technical help.


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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Pascal Sirand-Pugnet
    • 1
  • Cruz Santos
    • 1
  • Jacques Labarère
    • 1
    Email author
  1. 1.Laboratoire de Génétique et d′Amélioration des Champignons CultivésUniversity Victor Segalen, Bordeaux 2 INRA, C.R.A. de BordeauxVillenave d′Ornon CedexFrance

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