The Aa-Pri4 gene, specifically expressed during fruiting initiation in the Agrocybe aegerita complex, contains an unusual CT-rich leader intron within the 5′ uncoding region.
- 88 Downloads
The Aa1-Pri4 gene was cloned from the edible mushroom Agrocybe aegerita. The gene, specifically expressed during fruiting initiation, encodes a glycine-rich protein of 116 amino acids, with no homology to already known proteins. Homologous genes were amplified from two other strains belonging to the Agr. aegerita complex and originating from South-East Asia; and a comparison of the three genes revealed a high conservation of the coding sequences (72.8–97.8%). The PRI4 putative protein sequences were highly similar (87.5–100.0%); and all of them contained two protein kinase C sites, suggesting a potential supplementary regulation by phosphorylation at the protein level. The 5′ uncoding regions all presented a leader intron, very variable in sequence (45.7% identity), but with a high C+T content (74.5–79.0%). The presence of such CT-rich sequences previously described in the promoter of highly expressed fungal genes suggests that the leader intron of the Aa1-Pri4 gene could be involved in the high-level, stage-specific expression.
KeywordsBasidiomycota Fungi Fruiting initiation Leader intron CT-rich sequence
This research was supported by grants from the European Community (Fonds Européen de Développement Régional), the Conseil Scientifique de l′Université Victor Ségalen Bordeaux 2, Monsieur le Préfet de la Région Aquitaine Préfet de la Gironde (Fonds National d′Aménagement et de Développement du Territoire), the Conseil Régional d′Aquitaine and the Institut National de la Recherche Agronomique. We thank Prof. Jorge Wright and Dr. E. Alberto (Argentina), Dr. R. de Leon (Guatemala) and Dr. P. Pitakpaivan (Thailand) for kindly providing strains, and S. Chideh Soliman, C. Mulot and A.S. Scheuer for technical help.
- Esser K, Saleh F, Meinhardt F (1979) Genetic of fruit body production in higher basidiomycetes. 2. Monokaryotic and dikaryotic fruiting in Schizophyllum commune. Curr Genet 1:85–88Google Scholar
- Fu H, Kim SY, Park WD (1995) A potato Sus3 sucrose synthase gene contains a context-dependent 3′ element and a leader intron with both positive and negative tissue-specific effects. Plant Cell 7:1395–1403Google Scholar
- Gurr SJ, Unkles SE, Kinghorn JR (1987) The structure and organization of nuclear genes in filamentous fungi. In: Kinghorn JL (ed) Gene structure in eukaryotic microbes. IRL Press, London, pp 93–139Google Scholar
- Kim D, Azuma T (1999) Cloning of a gene specifically expressed during early stage of fruiting body formation in Flammulina velutipes. Korean J Mycol 27:187–190Google Scholar
- Kim D, et al (1999) Cloning and sequence analysis of a cDNA for the gene FVFD30, specifically expressed during fruiting body development in Flammulina velutipes. Mushroom Sci Biotechnol 7:95–99Google Scholar
- Meinhardt F, Esser K (1981) Genetic studies of the basidiomycete Agrocybe aegerita. 2. Genetic control of fruit body formation and its practical implications. Theor Appl Genet 60:265–268Google Scholar
- Noël T, Labarère J (1989) Isolation of DNA from Agrocybe aegerita for the construction of a genomic library in Escherichia coli. Mushroom Sci 12:187–201Google Scholar
- Raper CA, Raper JR, Miller RE (1972) Genetic analysis of the life cycle of Agaricus bisporus. Mycologia 64:108–117Google Scholar
- Sambrook J, Fristsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.Google Scholar
- Sirand-Pugnet P, Gonzalez P, Labarère J (2000) Phylogeny and evolution of mitochondrial sequences in mushrooms. In: Van Griensven LJLD (ed) Science and cultivation of edible fungi. Balkema, Rotterdam, pp 223–235Google Scholar
- White T, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis M, Gelfand D, Sninsky J, White T (eds) PCR protocols—a guide to methods and applications. Academic Press, New York, pp 315–322Google Scholar