Current Genetics

, Volume 44, Issue 3, pp 138–147

Cryptosporidium parvum Cpn60 targets a relict organelle

  • Christina E. Riordan
  • Jeffrey G. Ault
  • Susan G. Langreth
  • Janet S. Keithly
Research Article

Abstract

Chaperonin 60 (Cpn60) is a well-established marker protein for eukaryotic mitochondria and plastids. In order to determine whether the small double-membrane-bounded organelle posterior to the nucleus in the apicomplexan Cryptosporidium parvum is a mitochondrion, the Cpn60 gene of C. parvum sporozoites (CpCpn60) was analyzed and antibodies were generated for localization of the peptide. Sequence and phylogenetic analyses indicated that CpCpn60 is a mitochondrial isotype and that antibodies against it localize to the rough endoplasmic reticulum-enveloped remnant organelle of C. parvum sporozoites. These data show this organelle is of mitochondrial origin.

Keywords

Apicomplexan Relict mitochondrion Chaperonin 60 (Cpn60) 

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Christina E. Riordan
    • 1
    • 2
  • Jeffrey G. Ault
    • 1
  • Susan G. Langreth
    • 3
  • Janet S. Keithly
    • 1
    • 2
  1. 1.Wadsworth Center, New York State Department of HealthAxelrod Institute for Public HealthAlbanyUSA
  2. 2.Department of Biomedical SciencesSUNY Albany School of Public HealthAlbanyUSA
  3. 3.Department of Microbiology and ImmunologyUniformed Services University of the Health SciencesBethesdaUSA

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