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Current Genetics

, Volume 42, Issue 1, pp 43–50 | Cite as

Isomaltose formed by α-glucosidases triggers amylase induction in Aspergillus nidulans

  • Naoki Kato
  • Yuriko Murakoshi
  • Masashi Kato
  • Tetsuo KobayashiEmail author
  • Norihiro Tsukagoshi
Research Article

Abstract

Among various α-glucobioses examined, isomaltose was the most effective inducer for amylase synthesis in Aspergillus nidulans. Amylase induction by maltose was completely inhibited by addition of cast-anospermine or cycloheximide, while induction by isomaltose was not affected by the inhibitors, suggesting that amylase induction by maltose requires inducible α-glucosidases. Disruption of the α-glucosidase A gene (agdA), the α-glucosidase B gene (agdB), or both genes did not abolish maltose-dependent induction, although amylase production induced by maltose decreased about 2-fold in the agdA/agdB double disruptant, compared with that in the agdB disruptant at all concentrations tested. Upon induction by isomaltose, amylase synthesis was enhanced considerably in the agdB and agdA/agdB disruptants. Even at 3 nM, isomaltose induced amylase production in the double disruptant, supporting the suggestion that isomaltose is a physiological inducer for amylase. Therefore, maltose must be converted to isomaltose by α-glucosidases prior to triggering amylase synthesis, but no specific α-glucosidase is required for amylase induction by maltose. Probably any α-glucosidases having isomaltqse-forming activity, including AgdA and AgdB, may participate in amylase induction by maltose.

Keywords

Aspergillus nidulans α-Glucosidase Isomaltose Amylase induction 

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Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Naoki Kato
    • 1
  • Yuriko Murakoshi
    • 1
  • Masashi Kato
    • 1
  • Tetsuo Kobayashi
    • 1
    Email author
  • Norihiro Tsukagoshi
    • 1
  1. 1.Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural SciencesNagoya UniversityNagoyaJapan

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