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Current Genetics

, Volume 41, Issue 4, pp 275–281 | Cite as

Subtractive cloning of cDNA from Aspergillus oryzae differentially regulated between solid-state culture and liquid (submerged) culture

  • Takeshi Akao
  • Katsuya Gomi
  • Kuniyasu Goto
  • Naoto Okazaki
  • Osamu Akita
Research Article

Abstract.

In solid-state cultures (SC), Aspergillus oryzae shows characteristics such as high-level production and secretion of enzymes and hyphal differentiation with asexual development which are absent in liquid (submerged) culture (LC). It was predicted that many of the genes involved in the characteristics of A. oryzae in SC are differentially expressed between SC and LC. We generated two subtracted cDNA libraries with bi-directional cDNA subtractive hybridizations to isolate and identify such genes. Among them, we identified genes upregulated in or specific to SC, such as the AOS (A. oryzae SC-specific gene) series, and those downregulated or not expressed in SC, such as the AOL (A. oryzae LC-specific) series. Sequencing analyses revealed that the AOS series and the AOL series contain genes encoding extra- and intracellular enzymes and transport proteins. However, half were functionally unclassified by nucleotide sequences. Also, by expression profile, the AOS series comprised two groups. These gene products' molecular functions and physiological roles in SC await further investigation.

Aspergillus oryzae Solid-state culture cDNA subtraction Differential gene expression 

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Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Takeshi Akao
    • 1
  • Katsuya Gomi
    • 2
  • Kuniyasu Goto
    • 1
  • Naoto Okazaki
    • 1
  • Osamu Akita
    • 1
  1. 1.National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashi-Hiroshima 739-0046, Japan
  2. 2.Division of Life Science, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan

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