The properties of nanofiber scaffolds of polyurethane-Cinnamomum zeylanicum against pathogens of Pseudomonas aeruginosa and Staphylococcus aureus
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This study designed to investigate the properties of antibacterial nanofiber scaffolds of polyurethane-Cinnamomum zeylanicum against virulence gene expression inhibition of Pseudomonas aeruginosa and Staphylococcus aureus that are important in burn wounds. With attention to burn wound infections in hospitals and mortality increase in patients, it is necessary to design nanodressing. Clinical isolates were confirmed by biochemical and microbiological tests. DNA of isolates was extracted and PCR used to confirm the alp gene of P. aeruginosa and Pv gene of S. aureus. Polyurethane nanofiber and cinnamon polymers were used to prepare the scaffold under the electrospinning process. Infrared spectroscopy, electron microscopy, and mechanical tensile tests were used to confirm the scaffolds. The susceptibility testing and minimum inhibitory concentration of polyurethane-cinnamon nanofiber scaffold were determined against P. aeruginosa and S. aureus. For confirmation of polyurethane-cinnamon nanofiber scaffold were used the cytotoxicity test (MTT), FTIR, mechanical tensile test, and a scanning electron microscope. The expression of virulence genes was investigated using the real-time RT-PCR technique. The results of the susceptibility testing indicated that P. aeruginosa and S. aureus were susceptible to polyurethane-cinnamon nanofiber scaffold. The MTT, FTIR, mechanical tensile test, and SEM confirmed the different features of the polyurethane-cinnamon nanofiber scaffold. Results of real-time PCR demonstrated that the expression levels of p–v and alp genes after treatment decreased, respectively, 2.71- and 1.06-fold. Results indicated that the electrospun polyurethane-cinnamon nanofiber scaffold for the first time could inhibit both important pathogens of the hospital and the expression of the virulence genes. Considering the susceptibility of P. aeruginosa and S. aureus to and its inhibitory effect on an alp and p–v genes, this system could probably be a candidate in wound dressing for commercial purposes to burn healing and infection inhibition.
KeywordsPolyurethane-cinnamon Nanofiber Scaffold
We would like to thank our colleagues at BMSU, for their help.
DE conceived the project and designed the study. HH performed experiments. DE analyzed data and wrote the manuscript. All authors read and approved the final manuscript.
This work was supported by the authors.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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