Synthesis, characterization and biocompatible properties of novel silk fibroin/graphene oxide nanocomposite scaffolds for bone tissue engineering application
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Novel three-dimensional porous silk fibroin/graphene oxide (SF/GO) nanocomposite scaffolds with different graphene oxide (GO) concentrations were prepared by using the freeze-drying technique. The obtained SF/GO scaffolds were characterized by thermogravimetric analysis, X-ray diffraction, scanning electron microscopy, Brunauer–Emmett–Teller isotherm and Fourier transform infrared spectroscopy techniques. The water absorption, compressive properties, porosity, degradation, biomineralization capability, cell attachment and cell viability of the composite scaffolds were studied as well. Cytocompatibility of the scaffolds was studied in vitro by employing the methylthiazoletetrazolium assay. The results showed that the presence of graphene oxide nanoparticles throughout the fibroin matrix led to an increase in water uptake and mechanical properties; at the same time, the porosity of the scaffolds was decreased. The cell adhesion results also indicated that human osteoblast cells (MG-63) could adhere to the surface of SF/GO nanocomposites and develop on them. These suggest that SF/GO nanocomposite scaffolds may be a good candidate for bone tissue engineering applications.
KeywordsComposite scaffold Graphene oxide Silk fibroin Tissue engineering
The authors are thankful from Payame Noor University in Isfahan Research Council (Grant # 68424), and contributions from Isfahan University of Technology are gratefully acknowledged.
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