Sulfathiazole grafted PEG-PLL as pH-sensitive shielding system for cationic gene delivery
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Polycationic gene carriers, mPEG-b-PLL-g-succinyl sulfathiazole (denoted as PPS), were synthesized and characterized as a shielding system for cationic gene delivery. DNA/PEI/PPS ternary complexes were prepared by shielding PPS to DNA/PEI polyplex solution. PPS can shield the surface charge of DNA/PEI polyplexes from positive (27.7 mV) to negative (−24.6 mV), and the acidic condition should trigger the disassembly of ternary complex. The gene transfection efficiency of DNA/PEI/PPS was lower than DNA/PEI at pH 7.4, but at pH 6.0, it recovered to the same level as DNA/PEI. CLSM results showed the same trend as transfection at different pH. The suitable pH-triggered shielding/deshielding ability estimated that PPS should be a potential shielding system to be used in in vivo gene delivery.
KeywordsGene delivery Shielding system pH sensitive Biomaterials Nanoparticles
This work was financially supported by the National Natural Science Foundation of China (51203132, 51303173, 21474104 and 51403205), Natural Science Foundation of Guangdong Province, China (S2012040008070) and Foundation for Distinguished Young Talents in Higher Education of Guangdong, China (2012LYM_0093).
- 3.Walker GF, Ogris M, Wagner E (2008) Amine-reactive pyridylhydrazone-based PEG reagents for pH-reversible PEI polyplex shielding. Eur J Pharm Sci 34(4–5):309–320Google Scholar
- 18.Pittella F, Zhang M, Lee Y, Kim HJ, Tockary T, Osada K, Ishii T, Miyata K, Nishiyama N, Kataoka K (2011) Enhanced endosomal escape of siRNA-incorporating hybrid nanoparticles from calcium phosphate and PEG-block charge-conversional polymer for efficient gene knockdown with negligible cytotoxicity. Biomaterials 32(11):3106–3114CrossRefGoogle Scholar