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Polymer Bulletin

, Volume 73, Issue 12, pp 3503–3511 | Cite as

Sulfathiazole grafted PEG-PLL as pH-sensitive shielding system for cationic gene delivery

  • Jialiang XiaEmail author
  • Jie Chen
  • Lin Lin
  • Zhaopei Guo
  • Bing Han
  • Hongyan Yang
  • Zongcai Feng
  • Huayu TianEmail author
Original Paper

Abstract

Polycationic gene carriers, mPEG-b-PLL-g-succinyl sulfathiazole (denoted as PPS), were synthesized and characterized as a shielding system for cationic gene delivery. DNA/PEI/PPS ternary complexes were prepared by shielding PPS to DNA/PEI polyplex solution. PPS can shield the surface charge of DNA/PEI polyplexes from positive (27.7 mV) to negative (−24.6 mV), and the acidic condition should trigger the disassembly of ternary complex. The gene transfection efficiency of DNA/PEI/PPS was lower than DNA/PEI at pH 7.4, but at pH 6.0, it recovered to the same level as DNA/PEI. CLSM results showed the same trend as transfection at different pH. The suitable pH-triggered shielding/deshielding ability estimated that PPS should be a potential shielding system to be used in in vivo gene delivery.

Keywords

Gene delivery Shielding system pH sensitive Biomaterials Nanoparticles 

Notes

Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (51203132, 51303173, 21474104 and 51403205), Natural Science Foundation of Guangdong Province, China (S2012040008070) and Foundation for Distinguished Young Talents in Higher Education of Guangdong, China (2012LYM_0093).

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Jialiang Xia
    • 1
    • 3
    Email author
  • Jie Chen
    • 2
  • Lin Lin
    • 2
  • Zhaopei Guo
    • 2
  • Bing Han
    • 1
  • Hongyan Yang
    • 1
  • Zongcai Feng
    • 1
    • 3
  • Huayu Tian
    • 2
    Email author
  1. 1.School of Chemistry and Chemical EngineeringLingnan Normal UniversityZhanjiangChina
  2. 2.Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied ChemistryChinese Academy of ScienceChangchunChina
  3. 3.Research Center of Chemistry and MaterialsLingnan Normal University, Development Center for New Materials Engineering and Technology in Universities of GuangdongZhanjiangChina

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