Polymer Bulletin

, Volume 61, Issue 2, pp 147–156 | Cite as

Chelating polymer-based membranes. Preparation and use for metal ion scavenging and sorption of murine immunoglobulin G by immobilized Ni(II) ions

  • Kateřina KráčalíkováEmail author
  • Miroslav Bleha


Copolymers of 2-(2-hydroxyethoxy)ethyl methacrylate and ethylene dimethacrylate in the form of homogeneous membrane sheets were modified by chelating groups of iminodiacetic acid (IDA) in a two step reaction. The obtained sorbents showed high chelating capacity for Ni(II), Cu(II) and Fe(III) ions (up to 1.3 mmol/g). The potential use of immobilized Ni(II)-IDA complexes for sorption of murine immunoglobulin G by the immobilized metal affinity (IMA) method was tested. The sorption behaviour was characterized by a Langmuir-Freundlich adsorption isotherm. It was shown that the chelating membranes could be used for the sorption of low amounts of protein.


Ethyl Methacrylate Coir Fibre Iminodiacetic Acid Membrane Sheet Tosylchloride 
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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.Institute of Macromolecular Chemistry AS CR, v.v.i.Prague 6Czech Republic

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