Chelating polymer-based membranes. Preparation and use for metal ion scavenging and sorption of murine immunoglobulin G by immobilized Ni(II) ions
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Copolymers of 2-(2-hydroxyethoxy)ethyl methacrylate and ethylene dimethacrylate in the form of homogeneous membrane sheets were modified by chelating groups of iminodiacetic acid (IDA) in a two step reaction. The obtained sorbents showed high chelating capacity for Ni(II), Cu(II) and Fe(III) ions (up to 1.3 mmol/g). The potential use of immobilized Ni(II)-IDA complexes for sorption of murine immunoglobulin G by the immobilized metal affinity (IMA) method was tested. The sorption behaviour was characterized by a Langmuir-Freundlich adsorption isotherm. It was shown that the chelating membranes could be used for the sorption of low amounts of protein.
KeywordsEthyl Methacrylate Coir Fibre Iminodiacetic Acid Membrane Sheet Tosylchloride
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- 14.Kopeèek J, Jokl J, Lím D (1968) J Polym Sci C 16:3877–3889Google Scholar
- 16.Warburg O, Christian W (1941) Biochem Z 310:384–421Google Scholar
- 17.Kopeèek J, Lím D (1971) Collect Czech Chem Commun 36:2703–2707Google Scholar
- 18.Gatenholm P, Michálek J, Vacík J (1996) Macromol Symp 109:127–133Google Scholar