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Current Microbiology

, Volume 40, Issue 5, pp 310–314 | Cite as

Influence of Water Potential on Growth, Enzyme Secretion and In Vitro Enzyme Activities of Trichoderma harzianum at Different Temperatures

  • László Kredics
  • Zsuzsanna  Antal
  • László Manczinger

Abstract.

The influence of water potential on linear mycelial growth, secretion, and the in vitro activities of enzymes β-glucosidase, cellobiohydrolase, β-xylosidase, exochitinase, and chymotrypsin of Trichoderma harzianum strain T66 was studied at different temperatures. Nearly linear correlation was found between water potential and colony growth rate at both 25°C and 10°C, with higher growth rates at the higher temperature and higher water potentials. The amounts of enzyme secretion depended on the water potential and not on the quality of salt (NaCl or KCl) used as osmoticum. Enzyme activities were significantly affected by water potential. Significant enzyme activities were measured for most of the enzymes even at −14.800 megapascal (MPa), which is below the water potential where mycelial growth ceased. These results suggest the possibility of using mutants with improved xerotolerance for biocontrol purposes in soils with lower water potentials.

Keywords

Enzyme Enzyme Activity Water Potential High Growth Rate Trichoderma 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 2000

Authors and Affiliations

  • László Kredics
    • 1
  • Zsuzsanna  Antal
    • 2
  • László Manczinger
    • 1
  1. 1.Department of Microbiology, Attila József University, P.O. Box 533, H-6701 Szeged, Hungary HU
  2. 2.Research Group for Microbiology, Hungarian Academy of Sciences and Attila József University, P.O. Box 533, H-6701 Szeged, Hungary HU

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