Current Microbiology

, Volume 40, Issue 2, pp 96–100

Clarithromycin and Amoxicillin Susceptibility of Helicobacter pylori Strains Isolated from Adult Patients with Gastric or Duodenal Ulcer in Italy

  • Laura  Franzin
  • Marco  Pennazio
  • Daniela  Cabodi
  • Francesco  Paolo Rossini
  • Paolo  Gioannini

Abstract.

Helicobacter pylori strains, isolated from 100 gastric biopsies from 49 previously untreated adult patients with endoscopy and histology-confirmed gastric or duodenal ulcer, were tested for in vitro antimicrobial susceptibility. Strains were isolated from biopsies of 75.5% (37 of 49) patients before therapy and of 13.5% after therapy. Clarithromycin and amoxicillin susceptibility testing was performed on pretreatment and posttreatment strains by using the agar disk diffusion method and E-test, a quantitative technique for the minimal inhibitory concentration (MIC) determination. All strains (n = 53) were susceptible to amoxicillin by the two methods. Three strains of 34 (8.8%) patients were resistant to clarithromycin: two by both methods and one by E-test (MIC > 2 μg/ml). E-test, although more expensive than the disk diffusion method, is easy to perform and is a reliable method for testing H. pylori susceptibility to antimicrobial agents in the clinical microbiology laboratory.

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Copyright information

© Springer-Verlag New York Inc. 2000

Authors and Affiliations

  • Laura  Franzin
    • 1
  • Marco  Pennazio
    • 2
  • Daniela  Cabodi
    • 1
  • Francesco  Paolo Rossini
    • 2
  • Paolo  Gioannini
    • 1
  1. 1.Infectious Diseases Unit, University of Turin, Corso Svizzera 164, 10131 Turin, Italy IT
  2. 2.Department of Gastroenterology, San Giovanni A.S. Hospital, Via Cavour 31, 10123 Turin, Italy IT

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