Advertisement

Current Microbiology

, Volume 37, Issue 6, pp 380–386 | Cite as

Mass Balance Studies with 14C-Labeled 2,4,6-Trinitrotoluene (TNT) Mediated by an Anaerobic Desulfovibrio Species and an Aerobic Serratia Species

  • Oliver  Drzyzga
  • Dirk  Bruns-Nagel
  • Thomas  Gorontzy
  • Karl-Heinz  Blotevogel
  • Diethard  Gemsa
  • Eberhard  von Löw
Article

Abstract.

Investigations were carried out to evaluate the level of incorporation of radiolabeled 2,4,6-trinitrotoluene (TNT) and metabolites into the bacterial biomass of two different bacterial species after cometabolically mediated TNT transformation. Biotransformation experiments with 14C-TNT indicated that TNT was not mineralized; however, carbon derived from TNT became associated with the cells. It was found that more than 42% of the initially applied radiolabel was associated with the cell biomass after cometabolic 14C-TNT transformation with the strictly anerobic Desulfovibrio species strain SHV, whereas with the strictly aerobic Serratia plymuthica species strain B7, 32% of cell-associated 14C activity was measured. The remainder of the radiolabel was present in the supernatants of the liquid cultures in the form of different TNT metabolites. Under anoxic conditions with the Desulfovibrio species, TNT was ultimately transformed to 2,4,6-triaminotoluene (TAT) and both diaminonitrotoluene isomers, whereas under oxic conditions with the Serratia species, TNT was converted to hydroxylaminodinitrotoluenes and aminodinitrotoluenes, with 4-amino-2,6-dinitrotoluene (4ADNT) being the major end product. In both culture supernatants, small amounts of very polar, radiolabeled, but unidentified metabolites were detected. At the end of the experiments approximately 92% and 96% of the originally applied radioactivity was recovered in the studies with the Serratia and Desulfovibrio species, respectively.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag New York Inc. 1998

Authors and Affiliations

  • Oliver  Drzyzga
    • 1
  • Dirk  Bruns-Nagel
    • 1
  • Thomas  Gorontzy
    • 2
  • Karl-Heinz  Blotevogel
    • 2
  • Diethard  Gemsa
    • 1
  • Eberhard  von Löw
    • 1
  1. 1.Institut für Immunologie und Umwelthygiene, Bereich Umwelthygiene, Philipps-Universität Marburg, Pilgrimstein 2, D-35037 Marburg, Germany DE
  2. 2.Fachbereich Biologie, AG Mikrobiologie/Rüstungsaltlasten, Carl von Ossietzky-Universität Oldenburg, D-26111 Oldenburg, Germany DE

Personalised recommendations