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Current Microbiology

, Volume 36, Issue 2, pp 63–69 | Cite as

Sequence Analysis of the Internal Transcribed Spacer 2 (ITS2) from Yeast Species Within the Genus Candida

  • Timothy J.  Lott
  • Brian M.  Burns
  • Rosely  Zancope-Oliveira
  • Cheryl M.  Elie
  • Errol  Reiss

Abstract.

Nucleotide sequences of the internal transcribed spacer 2 (ITS2) regions were determined for 13 species within the genus Candida, representing a collection of those species pathogenic for humans. No two species had identical sequences and the sizes of ITS2 varied fourfold, representing an apparent continuous gradient of nucleotides. When present, sequence homologies were observed in the 5′ end of ITS2, and many species exhibited more limited homologies within three known conserved domains found in other yeasts. Cluster analysis of primary sequence revealed a concordance with a known taxonomic subfamily and suggests that certain species within the genus form a similar grouping. A majority of species exhibited similar presumptive RNA secondary structures, consistent with the hypothesis that these spacer regions are essential for correct processing of the 5.8S and 28S subunits.

Keywords

Nucleotide Sequence Analysis Nucleotide Sequence Cluster Analysis Secondary Structure 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1998

Authors and Affiliations

  • Timothy J.  Lott
    • 1
  • Brian M.  Burns
    • 2
  • Rosely  Zancope-Oliveira
    • 1
  • Cheryl M.  Elie
    • 1
  • Errol  Reiss
    • 1
  1. 1.Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention and Public Health Service, U.S. Department of Health and Human Services, Bldg. 5, B/12 mailstop G-11, 1600 Clifton Road NE, Atlanta, GA 30333, USA US
  2. 2.Department of Biology, Georgia State University, Atlanta, GA 30333, USA US

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