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Current Microbiology

, Volume 35 , Issue 1 , pp 22 –27 | Cite as

Construction of a Fibrobacter succinogenes Genomic Map and Demonstration of Diversity at the Genomic Level

  • Koretsugu  Ogata
  • Rustem I.  Aminov
  • Takafumi  Nagamine
  • Mutsumi  Sugiura
  • Kiyoshi  Tajima
  • Makoto  Mitsumori
  • Tsutomu  Sekizaki
  • Hiroshi  Kudo
  • Hajime  Minato
  • Yoshimi  Benno

Abstract.

The genomic cleavage map of the type strain Fibrobacter succinogenes S85 was constructed. The restriction enzymes AscI, AvrII, FseI, NotI, and SfiI generated DNA fragments of suitable size distribution that could be resolved by pulsed-field gel electrophoresis (PFGE). An average genome size of 3.6 Mb was obtained by summing the total fragment sizes. The linkages between the 15 AscI fragments of the genome were determined by combining two approaches: isolation of linking clones and cross-hybridization of restriction fragments. The genome of F. succinogenes was found to be represented by the single circular DNA molecule. Southern hybridization with specific probes allowed the eight genetic markers to be located on the restriction map. The genome of this bacterium contains at least three rRNA operons. PFGE of the other three strains of F. succinogenes gave estimated genome sizes close to that of the type strain. However, RFLP patterns of these strains generated by AscI digestion are completely different. Pairwise comparison of the genomic fragment distribution between the type strain and the three isolates showed a similarity level in the region of 14.3% to 31.3%. No fragment common to all of these F. succinogenes strains could be detected by PFGE. A marked degree of genomic heterogeneity among members of this species makes genomic RFLP a highly discriminatory and useful molecular typing tool for population studies.

Keywords

Type Strain Genome Size Typing Tool rRNA Operon RFLP Pattern 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1997

Authors and Affiliations

  • Koretsugu  Ogata
    • 1
  • Rustem I.  Aminov
    • 1
  • Takafumi  Nagamine
    • 1
  • Mutsumi  Sugiura
    • 1
  • Kiyoshi  Tajima
    • 1
  • Makoto  Mitsumori
    • 2
  • Tsutomu  Sekizaki
    • 2
  • Hiroshi  Kudo
    • 3
  • Hajime  Minato
    • 4
  • Yoshimi  Benno
    • 1
  1. 1.STAFF-Institute, Ippaizuka 446-1, Kamiyokoba, Tsukuba, Ibaraki 305, Japan JP
  2. 2.National Institute of Animal Health, Tsukuba 305, Japan JP
  3. 3.National Institute of Animal Industry, Tsukuba 305, Japan JP
  4. 4.Ibaraki University, Ami-machi 300-03, Japan JP

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