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Current Microbiology

, Volume 34 , Issue 4 , pp 244 –249 | Cite as

Serotyping and Enzyme Characterization of Pasteurella haemolytica and Pasteurella multocida Isolates Recovered from Pneumonic Lungs of Stressed Feeder Calves

  • Charles W.  Purdy
  • Russell H.  Raleigh
  • James K.  Collins
  • Jeffery L.  Watts
  • David C.  Straus

Abstract.

Ninety-one isolates of Pasteurella multocida (Pm) and 124 of Pasteurella haemolytica (Ph) were recovered from the lungs of calves that died of bovine respiratory tract disease (BRTD). Nine Pm enzyme profiles (A through I) and 9 Ph enzyme profiles (J through R) were determined for the Pasteurella isolates. The Pm isolates were relatively evenly divided among the enzyme profiles, with one exception, profile I. The Ph isolates were not evenly distributed among the profiles. Fifty of the 91 Pm isolates were serotyped. Forty-two Pm isolates were positive for capsule type A, and 8 were untypable. Five somatic type antigen profiles (3; 3,4; 3,7; 3,4,7; and 4) were identified among the 50 serotyped Pm isolates; one isolate was untypable. The Ph isolates were further divided through serotyping and grouped as follows: 74 (60%) Pasteurella haemolytica A1 (PhA1), 12 (10%) PhA2, 4 (3%) PhA5, and 34 (27%) PhA6. Eighty-one percent of the Ph serotypes were clustered in the M and N enzyme profile. The P enzyme profile was almost unique to PhA2 (8 of 12, 67% of PhA2 isolates). Results of this study indicate a need to collect more data on Ph serotypes at the state veterinary diagnostic laboratories.

Keywords

Enzyme Diagnostic Laboratory Enzyme Characterization Enzyme Profile Type Antigen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1997

Authors and Affiliations

  • Charles W.  Purdy
    • 1
  • Russell H.  Raleigh
    • 2
  • James K.  Collins
    • 3
  • Jeffery L.  Watts
    • 4
  • David C.  Straus
    • 5
  1. 1.USDA Agricultural Research Service, Conservation and Production Research Laboratory, Bushland, TX 79012, USA US
  2. 2.Texas A & M University, Veterinary Diagnostic Laboratory, Amarillo, TX 79106, USA US
  3. 3.Colorado State University, Diagnostic Laboratory, Fort Collins, CO 80523, USA US
  4. 4.Upjohn, Kalamazoo, MI 49001, USA US
  5. 5.Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA US

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