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Current Microbiology

, Volume 34 , Issue 4 , pp 199 –204 | Cite as

Gramicidin S Production by Bacillus brevis in Simulated Microgravity

  • A.  Fang
  • D.L.  Pierson
  • S.K.  Mishra
  • D.W.  Koenig
  • A.L.  Demain

Abstract.

In a continuing study of microbial secondary metabolism in simulated microgravity, we have examined gramicidin S (GS) production by Bacillus brevis strain Nagano in NASA High Aspect Rotating Vessels (HARVs), which are designed to simulate some aspects of microgravity. Growth and GS production were found to occur under simulated microgravity. When performance under simulated microgravity was compared with that under normal gravity conditions in the bioreactors, GS production was found to be unaffected by simulated microgravity. The repressive effect of glycerol in flask fermentations was not observed in the HARV. Thus the negative effect of glycerol on specific GS formation is dependent on shear and/or vessel geometry, not gravity.

Keywords

Fermentation Glycerol Bacillus Secondary Metabolism Normal Gravity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1997

Authors and Affiliations

  • A.  Fang
    • 1
  • D.L.  Pierson
    • 2
  • S.K.  Mishra
    • 3
  • D.W.  Koenig
    • 3
  • A.L.  Demain
    • 1
  1. 1.68-223, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA US
  2. 2.NASA/Johnson Space Center, Houston, TX 77058, USA US
  3. 3.KRUG Life Sciences, Inc., Houston, TX 77058, USA US

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