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Current Microbiology

, Volume 34 , Issue 3 , pp 167 –172 | Cite as

Choline and Glycine Betaine Uptake in Various Strains of Rhizobia Isolated from Nodules of Vicia faba var. major and Cicer arietinum L.: Modulation by Salt, Choline, and Glycine Betaine

  • Fatiha  Brhada
  • Marie-Christine  Poggi
  • Daniel  Le Rudulier

Abstract.

Two strains of Rhizobia isolated from nodules of Vicia faba var. major and one strain isolated from nodules of Cicer arietinum L. were characterized for salt resistance. The presence of 1 mM glycine betaine or choline in a minimal medium with added NaCl had a beneficial role on the growth of the three strains. Both molecules were found to be taken up by cells obtained at low osmolarity, and whereas glycine betaine uptake activity was stimulated significantly in cells grown in the presence of 0.15 M NaCl, choline uptake activity was strongly inhibited by salt in all tested strains. However, in cells grown with exogenous choline, the uptake inhibition exerted by salt was relieved, mainly in the strain isolated from nodules of C. arietinum L. On the basis of kinetics determinations, in control cells as well as in salt-stressed cells, only high-affinity activities were observed for glycine betaine and choline (apparent K m s between 3 and 18 μM). Periplasmic proteins that bound glycine betaine or choline were identified. In nondenaturing conditions, these proteins extracted from the various strains showed different electrophoretic mobility with always a less negative entire charge than the analogous proteins from Rhizobium meliloti.

Keywords

Choline Rhizobium Uptake Activity Glycine Betaine Vicia Faba 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1997

Authors and Affiliations

  • Fatiha  Brhada
    • 1
  • Marie-Christine  Poggi
    • 2
  • Daniel  Le Rudulier
    • 2
  1. 1.Laboratoire de Microbiologie et Biologie moléculaire, Département de Biologie, Faculté des Sciences, Université Mohammed V, BP 1014, Rabat, Maroc MA
  2. 2.Laboratoire de Biologie végétale et Microbiologie, URA CNRS 1114, Université de Nice-Sophia Antipolis, Parc Valrose, 06108 Nice Cédex, France FR

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