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Current Microbiology

, Volume 34 , Issue 2 , pp 103 –109 | Cite as

Isolation and Characterization of Dehalogenases from 2,2-Dichloropropionate-Degrading Soil Bacteria

  • Ricarda  Schwarze
  • Andre  Brokamp
  • Friedrich R.J.  Schmidt

Abstract.

Five bacterial strains were isolated from polluted soils capable of degrading 2,2-dichloropropionate. In crude extracts, dehalogenase activity against haloacetates and longer-chained 2-haloalkanoic acids could be detected. Results from activity staining indicated that all bacterial strains expressed a single dehalogenase. In further biochemical characterization, two types of D,L-specific 2-haloalkanoic acid dehalogenases were described, which are different from each other not only in molecular weight and electrophoretic mobility, but also in sensitivity towards thiol reagents. Dehalogenases of these strains have been shown to be inducible and are catalyzing halide hydrolysis with inversion of product configuration.

Keywords

Molecular Weight Hydrolysis Thiol Bacterial Strain Crude Extract 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1997

Authors and Affiliations

  • Ricarda  Schwarze
    • 1
  • Andre  Brokamp
    • 1
  • Friedrich R.J.  Schmidt
    • 1
  1. 1.Department of Microbiology, University Gesamthochschule Kassel, Faculty 19, Heinrich-Plett-Str. 40, D-34109 Kassel, Germany DE

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