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Effect of surfactants on cellulase production by Nectria catalinensis

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Abstract

The effect of different nonionic surfactants (Tween 80, Tween 20, Triton X-100) and polyethylene glycol (PEG 6000) was tested on cellulolytic enzyme system production. Tween 80 gave the highest yield of endoglucanase, exoglucanase, and cellobiase at the 20th day of growth, presumably by causing increased permeability of cell membranes and/or by promoting the release of cell-bound enzymes. Maximal yield of endoglucanase was achieved with 1.7mm Tween 80, whereas exoglucanase and cellobiase were at 0.85mm. In the same way, this compound increased fungal growth. On the other hand, Tween 20 and Triton X-100 inhibited growth and cellulolytic enzyme production. High yields of endoglucanase and exoglucanase were achieved with PEG 6000 in comparison with the control, presumably by increasing enzyme stability.

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Authors and Affiliations

  1. Laboratorio de Micología Experimental, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon II 4∘ Piso, 1428, Buenos Aires, Argentine

    Alejandro G. Pardo

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  1. Alejandro G. Pardo
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Pardo, A.G. Effect of surfactants on cellulase production by Nectria catalinensis . Current Microbiology 33, 275–278 (1996). https://doi.org/10.1007/s002849900113

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  • DOI: https://doi.org/10.1007/s002849900113

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