Advertisement

Current Microbiology

, Volume 32, Issue 4, pp 208–212 | Cite as

Pyrrolnitrin Production by an Enterobacter agglomerans Strain with a Broad Spectrum of Antagonistic Activity Towards Fungal and Bacterial Phytopathogens

  • Leonid  Chernin
  • Alexander  Brandis
  • Zafar  Ismailov
  • Ilan  Chet

Abstract.

Strain IC1270 of Enterobacter agglomerans has been previously described as a producer of a complex of chitinolytic enzymes and as an antagonist of many fungal phytopathogens [Chernin et al. (1995) Appl. Env. Microbiol. 61:1720–1726]. Here we show that this strain also produces an antibiotic that was purified by TLC and HPLC and identified by UV, IR, MS, and NMR analyses as pyrrolnitrin [3-chloro-4-(2′-nitro-3′-chlorophenyl)pyrrole]. The purified antibiotic is efficient against many phytopathogenic bacteria and fungi in vitro. This is the first piece of evidence showing that pyrrolnitrin can be produced by bacteria other than Pseudomonas and that one bacterial strain can simultaneously produce chitinolytic enzymes and pyrrolnitrin. The possible role of a combination of chitinases and pyrrolnitrin in antagonism is discussed.

Keywords

Enzyme HPLC Pseudomonas Bacterial Strain Broad Spectrum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag New York Inc. 1996

Authors and Affiliations

  • Leonid  Chernin
    • 1
  • Alexander  Brandis
    • 1
  • Zafar  Ismailov
    • 2
  • Ilan  Chet
    • 1
  1. 1.The Hebrew University of Jerusalem, Otto Warburg Center for Biotechnology in Agriculture, Faculty of Agriculture, P.O. Box 12, Rehovot 76100, Israel IL
  2. 2.Center “Bioengineering,” Russian Academy of Sciences, Vavilova St. 34/5, 117334, Moscow, Russia RU

Personalised recommendations