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Current Microbiology

, Volume 43, Issue 1, pp 11–16 | Cite as

The Replicon of the Cryptic Plasmid pSBO1 Isolated from Streptococcus bovis JB1

  • Mutsumi Nakamura
  • Koretsugu Ogata
  • Takafumi Nagamine
  • Kiyoshi Tajima
  • Hiroki Matsui
  • Yoshimi Benno

Abstract

The cryptic plasmid pSBO1 (3904 bp) was isolated from Streptococcus bovis JB1. pSBO1 contained an open reading frame (ORF) that is homologous to sequences encoding the replication protein (Rep) in pEFC1 (isolated from Enterococcus faecalis), pSK639 (Staphylococcus epidermidis), pLA103 (Lactobacillus acidophilus), and pUCL287 (Tetragenococcus halophila). In addition, four 22-bp direct repeats (DRs) were located upstream of the putative replication gene (rep) of pSBO1. Recombinant plasmids (pSBE10 and pSBE11) containing the DRs and putative rep of pSBO1 replicated in S. bovis 12-U-1 and no8 strains. This result indicates that the putative rep encoded Rep and that the replicon of pSBO1 contained the DRs and the rep. Gel shift assays showed that the Rep of pSBO1 bound the 22-bp DRs.

Keywords

Lactobacillus Staphylococcus Recombinant Plasmid Enterococcus Faecalis Direct Repeat 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 2001

Authors and Affiliations

  • Mutsumi Nakamura
    • 1
  • Koretsugu Ogata
    • 2
  • Takafumi Nagamine
    • 1
  • Kiyoshi Tajima
    • 1
  • Hiroki Matsui
    • 1
  • Yoshimi Benno
    • 1
  1. 1.Rumen Microbiology Research Team, STAFF-Institute, 446-1 Ippaizuka, Kamiyokoba, Tsukuba, Ibaraki 305-0854, JapanJP
  2. 2.Analytical Instruments Division, Genomic Research Department, Shimadzu Corporation, 1, Nishinokyo-kuwabaracho, Nakagyo-ku, Kyoto 604-8511, JapanJP

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