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Current Microbiology

, Volume 42, Issue 5, pp 350–352 | Cite as

Cloning and Sequence Analysis of the Endopolygalacturonase Gene from the Pitch Canker Fungus, Fusarium circinatum

  • Percy M. Chimwamurombe
  • Brenda D. Wingfield
  • Anna-Maria Botha
  • Michael J. Wingfield

Abstract

The fungus Fusarium circinatum causes pitch canker disease on mature pine trees and root rot and damping-off of pine seedlings. Endopolygalacturonases (endoPGs) play a major role during penetration of plants by fungi. Digestion of the pectic polysaccharides in the plant primary cell walls is one of the earliest functions of endoPGs during infection. The research objective was to clone and characterize the gene encoding endopolygalacturonase in F. circinatum. A 970-bp DNA fragment was cloned by using degenerate PCR amplification from F. circinatum DNA. Sequence data for this fragment were used to design specific primers for use in genome walking to amplify and sequence the remaining portion of the F. circinatum endoPG gene (Fcpg). The amino acid sequence predicted from this gene showed 90% and 87% similarity to Fusarium oxysporum and Fusarium moniliforme endoPGs, respectively.

Keywords

Fusarium Fusarium Oxysporum Primary Cell Wall Early Function Fusarium Moniliforme 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 2001

Authors and Affiliations

  • Percy M. Chimwamurombe
    • 1
  • Brenda D. Wingfield
    • 1
  • Anna-Maria Botha
    • 2
  • Michael J. Wingfield
    • 3
  1. 1.Forest Molecular Biology Co-operative Programme, Department of Genetics, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002, South AfricaZA
  2. 2.Departments of Genetics and Botany, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002, South AfricaZA
  3. 3.Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria 0002, South AfricaZA

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