Current Microbiology

, Volume 41, Issue 3, pp 161–166

Current Status of Helicobacter Contamination of Laboratory Mice, Rats, Gerbils, and House Musk Shrews in Japan

  • Kazuo Goto
  • Hiroaki Ohashi
  • Akira Takakura
  • Toshio Itoh

DOI: 10.1007/s002840010111

Cite this article as:
Goto, K., Ohashi, H., Takakura, A. et al. Curr Microbiol (2000) 41: 161. doi:10.1007/s002840010111

Abstract

The current status of Helicobacter contamination of laboratory mice, rats, gerbils, and house musk shrews from 47, 10, 4, and 3 colonies, respectively, in Japan was studied. Helicobacter was detected by reverse transcription (RT)-nested polymerase chain reaction (PCR) with Helicobacter genus-specific primers by using feces obtained from the animals. H. hepaticus, H. muridarum, H. bilis, H. rodentium, “Flexispira rappini”, and “H. suncus” were identified with species-specific primers. Other species of Helicobacter were identified by sequencing of PCR products amplified with genus-specific primers. For mice, H. hepaticus, H. bilis, H. rodentium including H. rodentium-like organism, “H. typhlonicus”-like organism, and H. westmeadii-like organism were detected from 12 (25.5%), 1 (2.1%), 18 (38.3%), 1 (2.1%), and 1 (2.1%) colonies, respectively. Forty-seven (5.7%) mice from six (12.8%) colonies were contaminated with both H. hepaticus and H. rodentium. H. rodentium was detected in rats from three (30.0%) colonies. In gerbils, H. hepaticus was detected in three (75.0%) colonies, but other species of Helicobacter were not detected. In house musk shrews, “H. suncus” was detected in animals from two (66.7%) colonies. Visible lesions in the liver, which showed multiple pale to white foci, were observed in 6/42, 5/174, and 1/21 mice infected with H. hepaticus, H. rodentium, and H. hepaticus/H. rodentium, respectively, and 1/14 gerbils infected with H. hepaticus. The results suggest the prevalence of these species of Helicobacter in mice, rats, gerbils, and house musk shrews in Japan.

Copyright information

© Springer-Verlag New York Inc. 2000

Authors and Affiliations

  • Kazuo Goto
    • 1
  • Hiroaki Ohashi
    • 1
  • Akira Takakura
    • 1
  • Toshio Itoh
    • 1
  1. 1.Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasakishi, Kanagawa, 216-0001, JapanJP

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